NDLI: A High-Resolution NMR Approach Combined to MALDI-TOF-MS to Estimate the Positional Distribution of Acyl-Linked Unsaturated Fatty Acids in Triacylglycerols

Content Provider	Springer Nature Link
Author	Meusel, Andrej Popkova, Yulia Dannenberger, Dirk Schiller, Jürgen
Copyright Year	2017
Abstract	Triacylglycerols (TAGs) are the most abundant constituents of vegetable oils and adipose tissues. TAG analysis is even, nowadays, challenging because the presence of three (often different) fatty acyl residues leads to many different, regioisomeric species. While the overall fatty acyl composition of TAG can be determined by gas chromatography/mass spectrometry (GC/MS), all positional information is completely lost by this approach. Therefore, GC/MS is often replaced or combined with LC/MS that provides information (by MS/MS) which fatty acyl residues are present in a given TAG and sometimes also about the location at the glycerol backbone. Since TAGs are normally available in huge amounts, the comparably low sensitivity of NMR is not a major issue. We show that high-resolution $^{13}$C NMR spectroscopy is an excellent method to determine the overall fatty acyl composition and the positions of the related (unsaturated) fatty acyl residues in a given TAG molecule. These data will be compared with MALDI-TOF MS data of the same vegetable oil samples. Although the $^{13}$C NMR method is particularly applicable to unsaturated TAG, this approach can be regarded as a significant methodological progress in this field.
Starting Page	2497
Ending Page	2506
Page Count	10
File Format	PDF
ISSN	19369751
Journal	Food Analytical Methods
Volume Number	10
Issue Number	7
e-ISSN	1936976X
Language	English
Publisher	Springer US
Publisher Date	2017-01-25
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Triacylglycerols Regioisomers Vegetable oils $^{1}$H NMR $^{13}$C NMR spectroscopy MALDI-TOF mass spectrometry Food Science Chemistry/Food Science Microbiology Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Safety Research Safety, Risk, Reliability and Quality Applied Microbiology and Biotechnology Food Science

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