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A New Real-Time PCR Assay for the Specific Detection of Salmonella spp. Targeting the bipA Gene

Content Provider	Springer Nature Link
Author	Calvó, Laia Martínez Planells, Asunción Pardos Bosch, Joana Garcia Gil, L. Jesús
Copyright Year	2007
Abstract	A straightforward real-time polymerase chain reaction (PCR)-based assay was designed and evaluated for the detection of Salmonella spp. in food and water samples. This new assay is based on the specific detection of the bipA gene of Salmonella, which encodes a protein of the guanosine triphosphate (GTP)-binding elongation family that displays global modulating properties, by regulating a wide variety of downstream processes. The new method correctly identified all 48 Salmonella strains used in the inclusivity test, and did not detect all 30 non-Salmonella species tested. The method was evaluated by analyzing 120 diverse food and water samples enriched in buffered peptone water. The bipA-based real-time PCR assay showed 100% efficiency, sensitivity, and specificity compared to the invA-based method previously published, which was developed as a part of a European project for the standardization of PCR methods in food microbiology. The assay includes an independent internal amplification control (IAC) in each reaction to control false negative results.
Starting Page	236
Ending Page	242
Page Count	7
File Format	PDF
ISSN	19369751
Journal	Food Analytical Methods
Volume Number	1
Issue Number	4
e-ISSN	1936976X
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-03-28
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Salmonella spp. BipA Real-time PCR Food Analysis Analytical Chemistry Microbiology Chemistry/Food Science Food Science
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Safety Research Safety, Risk, Reliability and Quality Applied Microbiology and Biotechnology Food Science

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