NDLI: Genetic Diversity of Twelve Switchgrass Populations Using Molecular and Morphological Markers

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Genetic Diversity of Twelve Switchgrass Populations Using Molecular and Morphological Markers

Content Provider	Springer Nature Link
Author	Cortese, Laura M. Honig, Joshua Miller, Chris Bos, Stacy A.
Copyright Year	2010
Abstract	Switchgrass (Panicum virgatum L.) is a warm season, C$_{4}$ perennial grass native to most of North America with numerous applications, including use as a bioenergy feedstock species. To date, no studies on genetic diversity in switchgrass have been conducted that use both molecular and morphological markers. The objectives of this study were to assess genetic diversity and determine differences among and between 12 switchgrass populations grown in New Jersey by examining both morphological and molecular characteristics, and to determine whether morphological, molecular, and/or combined data sets can detect ecotype and/or geographical differences at the population level. Twelve plants from each population were characterized with 16 switchgrass expressed sequence tag-simple sequence repeat markers (EST-SSRs) and seven morphological characters. Data was analyzed using GenAlEx and Unweighted Pair-Group Method of Averages (UPGMA) cluster analysis. Most (64%) of the molecular variation in switchgrass populations exists among individuals within populations, with lesser amounts between populations (36%). Upland and lowland populations were distinguished in all three data sets. Some eastern US and midwestern US populations were distinct in all three data sets. Similarities were observed between all three data sets indicating molecular markers may be useful for identifying morphological differences or other adaptive traits. The combined data set was the most useful in differentiating populations based on geography and found separation between midwestern and eastern upland populations. The results indicate that the combination of morphological and molecular markers may be useful in future applications such as genetic diversity studies, plant variety protection, cultivar identification, and/or identifying geographic origin.
Starting Page	262
Ending Page	271
Page Count	10
File Format	PDF
ISSN	19391234
Journal	BioEnergy Research
Volume Number	3
Issue Number	3
e-ISSN	19391242
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-01-27
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ecotype Genetic diversity Lowland Switchgrass Upland Wood Science & Technology Plant Ecology Plant Breeding/Biotechnology Plant Genetics & Genomics Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Renewable Energy, Sustainability and the Environment Agronomy and Crop Science

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