NDLI: SRAP Polymorphisms Associated to Cell Wall Degradability in Lignified Stems of Alfalfa

Content Provider	Springer Nature Link
Author	Dubé, Marie Pier Castonguay, Yves Duceppe, Marc Olivier Bertrand, Annick Michaud, Réal
Copyright Year	2012
Abstract	The identification of DNA polymorphisms associated to increased cell wall (CW) degradability could accelerate the development of alfalfa (Medicago sativa L.) cultivars with superior ethanol conversion yields. Genotypes with high (D+) or low (D−) CW degradability were recently identified within a biomass-type and three winter-hardy-type populations using near-infrared reflectance spectroscopy (NIRS) prediction of CW glucose released by enzyme saccharification. In this report, we used sequence-related amplified polymorphism to search for DNA variations associated to differences in enzyme-released glucose. A bulk segregant analysis (BSA) of pooled DNA samples (20 plants/bulk) from D+, D−, and randomly chosen genotypes uncovered polymorphisms associated to CW degradability. Polymorphisms that increase or decrease in intensity between D+ and D− bulks indicated the presence of genomic regions with either positive or negative effects on CW degradability. A primer pair (Me4-R14) generated a fragment, which increased in intensity in the D+ bulk of the biomass-type population. Conversely, the amplification of that fragment declined in the D+ bulks of the winter-hardy-type populations. Interestingly, these populations differ in their degradability. Assessment of the genotypic occurrence of this fragment confirmed that polymorphism detected with BSA reflects changes in the frequency of occurrence within populations. Sequence analysis of the Me4-R14 fragment revealed homologies with sequences from Medicago truncatula, a model species for legumes with documented synteny with M. sativa. Our results show that genomic regions associated to CW degradability can be identified using the combination of BSA of genotypes with contrasted degradability and a PCR-based amplification technique.
Starting Page	644
Ending Page	650
Page Count	7
File Format	PDF
ISSN	19391234
Journal	BioEnergy Research
Volume Number	6
Issue Number	2
e-ISSN	19391242
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-12-11
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Alfalfa Bioethanol Cell wall degradability DNA polymorphism Sequence-related amplified polymorphism Plant Sciences Plant Genetics & Genomics Plant Breeding/Biotechnology Plant Ecology Wood Science & Technology
Content Type	Text
Resource Type	Article
Subject	Renewable Energy, Sustainability and the Environment Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Assessment of Genetic Variability of Cell Wall Degradability for the Selection of Alfalfa with Improved Saccharification Efficiency

Medicago truncatula as a Model for Dicot Cell Wall Development

Sequence-related amplified polymorphism (SRAP) molecular marker system and its applications in crop improvement

Genetic diversity and population structure of four Iranian alfalfa populations revealed by sequence-related amplified polymorphism (SRAP) markers

Eucalyptus Cell Wall Architecture: Clues for Lignocellulosic Biomass Deconstruction

Quantitative Sustainability Assessment of Seaweed Biomass as Bioethanol Feedstock

Sugarcane as a Bioenergy Source: History, Performance, and Perspectives for Second-Generation Bioethanol

Composition and Structure of Sugarcane Cell Wall Polysaccharides: Implications for Second-Generation Bioethanol Production

A Survey of Databases for Analysis of Plant Cell Wall-Related Enzymes

SRAP Polymorphisms Associated to Cell Wall Degradability in Lignified Stems of Alfalfa

Similar Documents

Assessment of Genetic Variability of Cell Wall Degradability for the Selection of Alfalfa with Improved Saccharification Efficiency

Medicago truncatula as a Model for Dicot Cell Wall Development

Sequence-related amplified polymorphism (SRAP) molecular marker system and its applications in crop improvement

Genetic diversity and population structure of four Iranian alfalfa populations revealed by sequence-related amplified polymorphism (SRAP) markers

Eucalyptus Cell Wall Architecture: Clues for Lignocellulosic Biomass Deconstruction

Quantitative Sustainability Assessment of Seaweed Biomass as Bioethanol Feedstock

Sugarcane as a Bioenergy Source: History, Performance, and Perspectives for Second-Generation Bioethanol

Composition and Structure of Sugarcane Cell Wall Polysaccharides: Implications for Second-Generation Bioethanol Production

A Survey of Databases for Analysis of Plant Cell Wall-Related Enzymes

SRAP Polymorphisms Associated to Cell Wall Degradability in Lignified Stems of Alfalfa