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| Content Provider | Springer Nature Link |
|---|---|
| Author | Dwivedi, Vatsala Kumari, Kirti Gupta, Sanjay Kumar Kumari, Rekha Tripathi, Charu Lata, Pushp Niharika, Neha Singh, Amit Kumar Kumar, Roshan Nigam, Aeshna Garg, Nidhi Lal, Rup |
| Copyright Year | 2015 |
| Abstract | A Gram negative, yellow pigmented, rod shaped bacterium designated as RL$^{T}$ was isolated from a hot water spring (90–98 °C) located at Manikaran in Northern India. The isolate grows at 60–80 °C (optimum, 70 °C) and at pH 7.0–9.0 (optimum pH 7.2). Phylogenetic analysis of 16S rRNA gene sequences and levels of DNA–DNA relatedness together indicate that the new isolate represents a novel species of the genus Thermus with closest affinity to Thermus thermophilus HB8$^{T}$ (99.5 %) followed by Thermus arciformis (96.4 %). A comparative analysis of partial sequences of housekeeping genes (HKG) further revealed that strain RL$^{T}$ is a novel species belonging to the genus Thermus. The melting G+C content of strain RL$^{T}$ was calculated as 68.7 mol%. The DNA–DNA relatedness value of strain RL$^{T}$ with its nearest neighbours (>97 %) was found to be less than 70 % indicating that strain RL$^{T}$ represents a novel species of the genus Thermus. MK-8 was the predominant respiratory quinone. The presence of characteristic phospholipid and glycolipid further confirmed that strain RL$^{T}$ belongs to the genus Thermus. The predominant fatty acids of strain RL$^{T}$ were iso-C$_{17:0}$ (23.67 %) and iso-C$_{15:0}$ (24.50 %). The results obtained after DNA–DNA hybridization, biochemical and physiological tests clearly distinguished strain RL$^{T}$ from its closely related species. Thus, strain RL$^{T}$ represents a novel species of the genus Thermus for which the name Thermus parvatiensis is proposed (=DSM 21745$^{T}$= MTCC 8932$^{T}$). |
| Starting Page | 357 |
| Ending Page | 365 |
| Page Count | 9 |
| File Format | |
| ISSN | 00468991 |
| Journal | Indian Journal of Microbiology |
| Volume Number | 55 |
| Issue Number | 4 |
| e-ISSN | 09737715 |
| Language | English |
| Publisher | Springer India |
| Publisher Date | 2015-06-14 |
| Publisher Institution | Association of Microbiologists of India |
| Publisher Place | New Delhi |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Hot spring Thermus parvatiensis Manikaran Thermophile Microbiology Medical Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology |
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