NDLI: Regulation of Saccharomyces cerevisiae Plasma membrane H$^{+}$-ATPase (Pma1) by Dextrose and Hsp30 during Exposure to Thermal Stress

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Indian Journal of Microbiology : Volume 51, Issue 1, January 2011

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Regulation of Saccharomyces cerevisiae Plasma membrane H$^{+}$-ATPase (Pma1) by Dextrose and Hsp30 during Exposure to Thermal Stress

Content Provider	Springer Nature Link
Author	Meena, Ramesh C. Thakur, Suresh Chakrabarti, Amitabha
Copyright Year	2011
Abstract	Pma1p is an essential plasma membrane H$^{+}$-pump in Saccharomyces cerevisiae that pumps out H$^{+}$ at the expense of cellular ATP. Its activity is induced by glucose at 30°C and is inhibited by Hsp30 during exposure to heat shock conditions. To further investigate the regulation of Pma1 function by glucose and Hsp30 during exposure to thermal stress, we estimated Pma1 activity, its protein levels and ser-phosphorylation status in membrane fractions isolated from BY4741 and hsp30Δ cells grown in dextrose and sorbitol at 30°C, and following exposure at 40°C for 30 min. Our results demonstrate that Pma1 activity and protein levels were reduced in Hsp30$^{+}$ cells following exposure to thermal stress in dextrose media. The above was not observed in hsp30Δ cells wherein Pma1 activity did not decrease following exposure to similar conditions. Although Pma1p levels decreased in heat-shocked hsp30Δ cells, it was lower compared to that observed in Hsp30$^{+}$ cells. Total ser-phosphorylation of Pma1 also showed a decrease following exposure to heat shock condition in dextrose media in both BY4741 and hsp30Δ cells. Its levels were also reduced in BY4741 cells upon heat shock treatment in sorbitol unlike that observed in hsp30Δ cells wherein it was increased. Taken together the above indicate that heat shock induced reduction in Pma1 activity and protein levels in dextrose media required Hsp30. To examine functional interactions between dextrose utilization, Hsp30 and the regulation of various aspects of Pma1, we determined if dextrose regulated other functions attributed to Hsp30. Results demonstrate that the deletion of HSP30 rendered cells dependent on dextrose utilization for survival during exposure to lethal heat stress. Our study has hence been able to establish a functional relationship between glucose utilization, Hsp30 function and the regulation of Pma1 activity. Finally, since the deletion of HSP30 renders Pma1p levels and its activity unresponsive to thermal stress in dextrose media, we concluded that Hsp30 is necessary to maintain Pma1 in a regulation competent conformation. Hsp30 may thus act as a chaperone in the S. cerevisiae plasma membrane.
Starting Page	153
Ending Page	158
Page Count	6
File Format	PDF
ISSN	00468991
Journal	Indian Journal of Microbiology
Volume Number	51
Issue Number	2
e-ISSN	09737715
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-01-25
Publisher Institution	Association of Microbiologists of India
Publisher Place	India
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Saccharomyces cerevisiae Pma1 Dextrose Hsp30 Chaperone Microbiology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Microbiology

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Regulation of Saccharomyces cerevisiae Plasma membrane $H^{+}-ATPase$ (Pma1) by Dextrose and Hsp30 during Exposure to Thermal Stress

Regulation of Saccharomyces cerevisiae Plasma membrane H+-ATPase (Pma1) by Dextrose and Hsp30 during Exposure to Thermal Stress

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Regulation of Saccharomyces cerevisiae Plasma membrane H$^{+}$-ATPase (Pma1) by Dextrose and Hsp30 during Exposure to Thermal Stress

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