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| Content Provider | Springer Nature Link |
|---|---|
| Author | Kitti, Thawatchai Thummeepak, Rapee Thanwisai, Aunchalee Boonyodying, Kamala Kunthalert, Duangkamol Ritvirool, Pannika Sitthisak, Sutthirat |
| Copyright Year | 2014 |
| Abstract | Acinetobacter baumannii is an opportunistic pathogen that exists in hospital environments. The emergence of multidrug resistant A. baumannii (MDRAB) has been reported worldwide. It is necessary to find a novel and effective treatment for MDRAB infection. In this study, three bacteriophages, designated as ØABP-01, ØABP-02 and ØABP-04 were selected for analysis. Transmission electron microscopy showed that bacteriophage ØABP-01 belonged to the Podoviridae family and bacteriophage ØABP-02 and ØABP-04 are classified into the family Myoviridae. ØABP-01 had the widest host range. ØABP-01, ØABP-02 and ØABP-04 exhibited a latent period of 15, 20 and 20 min. The burst sizes of the three bacteriophages were 110, 120 and 150 PFU/cell. DNA restriction analysis using EcoRI, HindIII, PstI, SphI, BamHI and SmaI showed different DNA fragment patterns between the three bacteriophages. ØABP-01 and ØABP-04 was positive for the endolysin gene as determined by PCR. In conclusion, bacteriophage ØABP-01 showed broad host-specificity, good lytic activity and a short latency period, making it an appropriate candidate for studying the control and diagnosis associated with MDRAB infections. |
| Starting Page | 383 |
| Ending Page | 388 |
| Page Count | 6 |
| File Format | |
| ISSN | 00468991 |
| Journal | Indian Journal of Microbiology |
| Volume Number | 54 |
| Issue Number | 4 |
| e-ISSN | 09737715 |
| Language | English |
| Publisher | Springer India |
| Publisher Date | 2014-05-21 |
| Publisher Institution | Association of Microbiologists of India |
| Publisher Place | India |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Acinetobacter baumannii Bacteriophage Endolysin Microbiology Medical Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology |
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