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| Content Provider | Springer Nature Link |
|---|---|
| Author | Saurav, Gunjan Kumar Daimei, Guisuibou Rana, Vipin Singh Popli, Sonam Rajagopal, Raman |
| Copyright Year | 2016 |
| Abstract | Thrips palmi Karny is a globally distributed polyphagous agricultural pest. It causes huge economic loss by its biological behaviors like feeding, reproduction and transmission of tospoviruses. Since T. palmi shows close morphological similarities with other thrips species, we employed mitochondrial cytochrome oxidase 1 (mtCO1) gene as a molecular marker. BLAST analysis of this sequence helped us to identify the collected specimen as T. palmi. We observed the female to male ratio of about 3:1 from collected samples and suspected the presence of Wolbachia. The presence of Wolbachia was detected by PCR using genus specific primers of 16S rRNA gene. Further confirmation of Wolbachia strain was achieved by conducting PCR amplification of three ubiquitous genes ftsZ, gatB and groEL. A phylogenetic tree was constructed with concatenated sequences of ftsZ and gatB gene to assign supergroup to Wolbachia. Finally, we localized Wolbachia in abdominal region of the insect using fluorescent in situ hybridization with the help of confocal microscope. Our result confirmed the presence of Wolbachia supergroup B strain for the first time in T. palmi. |
| Starting Page | 167 |
| Ending Page | 171 |
| Page Count | 5 |
| File Format | |
| ISSN | 00468991 |
| Journal | Indian Journal of Microbiology |
| Volume Number | 56 |
| Issue Number | 2 |
| e-ISSN | 09737715 |
| Language | English |
| Publisher | Springer India |
| Publisher Date | 2016-02-13 |
| Publisher Institution | Association of Microbiologists of India |
| Publisher Place | New Delhi |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | mtCO1 gene Ubiquitous genes 16S rRNA gene PCR FISH Microbiology Medical Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology |
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