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Indian Journal of Microbiology : Volume 49, Issue 2, June 2009

Indian Journal of Microbiology : Volume 49, Issue 1, March 2009

Indian Journal of Microbiology : Volume 48

Indian Journal of Microbiology : Volume 47

Simultaneous detection of pathogenic B. cereus, S. aureus and L. monocytogenes by multiplex PCR

Content Provider	Springer Nature Link
Author	Kumar, T. D. Kalyan Murali, H. S. Batra, H. V.
Copyright Year	2009
Abstract	Three important foodborne pathogens, Bacillus cereus, Listeria monocytogenes and Staphylococcus aureus are of major concern for food safety in terms of frequency and seriousness of the disease. The occurrence these three important pathogens and their coexistence in food matrices are predominant. Moreover, symptoms associated with B. cereus and S. aureus food poisoning not only closely resembles each other but can also be overlapping with other foodborne infections. In this context, detection of these three pathogens simultaneously in food samples by a single multiplex PCR (mPCR) would have advantages in terms of rapidity and cost saving, when compared with single organism specific PCRs. mPCR has been standardized by targeting three major diarrheal enterotoxin genes hbl A, cyt K and nhe A of B. cereus, virulence associated nuc and Ent B genes of S. aureus and virulence associated hly and iap genes of L. monocytogenes along with internal amplification control (IAC). The results showed that mPCR accurately identified all the three organisms individually or in combination without non-specificity. The mPCR was able to detect as low as 10 to 100 organisms per ml of growth following overnight enrichment of spiked food samples (vegetable biriyani and milk) and their presence in naturally contaminated samples also. The high throughput and cost effective multiplex PCR method developed in this study could provide a powerful tool for simultaneous, rapid and reliable detection of B. cereus, S. aureus and L. monocytogenes in food samples.
Starting Page	283
Ending Page	289
Page Count	7
File Format	PDF
ISSN	00468991
Journal	Indian Journal of Microbiology
Volume Number	49
Issue Number	3
e-ISSN	09737715
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-06-05
Publisher Institution	Association of Microbiologists of India
Publisher Place	India
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bacillus cereus Listeria monocytogenes Staphylococcus aureus mPCR IAC Pathogens Medical Microbiology Microbiology
Content Type	Text
Resource Type	Article
Subject	Microbiology

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