NDLI: Feedback-controlled electro-kinetic traps for single-molecule spectroscopy

Content Provider	Springer Nature Link
Author	KUMBAKHAR, MANOJ HÄHNEL, DIRK GREGOR, INGO ENDERLEIN, JÖRG
Copyright Year	2014
Abstract	A principal limitation of single-molecule spectroscopy in solution is the diffusion-limited residence time of a given molecule within the detection volume. A common solution to this problem is to immobilize molecules of interest on a passivated glass surface for extending the observation time to obtain reliable data statistics. However, surface tethering of molecules often introduces artifacts, particularly when studying the structural dynamics of biomolecules. To circumvent this limitation, we investigated alternative ways to extend single-molecule observation times in solution without surface immobilization. Among various possibilities, the so-called anti-Brownian electro-kinetic trap (or ABEL trap) seems best suited to achieve this goal. The essential part of that trap is a feedback-controlled electro-kinetic steering of a molecule’s position in reaction to its diffusive Brownian motion which is monitored by fluorescence, thus keeping the molecule within a sub-micron sized detection volume. Fluorescence trace recordings of over thousands of milliseconds duration on individual dye molecules within an ABEL trap have been reported. In this short review, we shall briefly discuss the principle and some results of ABEL trapping of individual molecules with possible extensions to future works.
Starting Page	121
Ending Page	134
Page Count	14
File Format	PDF
ISSN	03044289
Journal	Pramana
Volume Number	82
Issue Number	1
e-ISSN	09737111
Language	English
Publisher	Springer India
Publisher Date	2014-01-11
Publisher Place	India
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Single-molecule fluorescence spectroscopy active trapping of molecules anti-Brownian electro-kinetic trap Physics Astronomy, Observations and Techniques Astrophysics and Astroparticles
Content Type	Text
Resource Type	Article
Subject	Physics and Astronomy

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Quantum logic gates using coherent population trapping states

Compact fibre-based coherent anti-Stokes Raman scattering spectroscopy and interferometric coherent anti-Stokes Raman scattering from a single femtosecond fibre-laser oscillator

Optimization of transfer of laser-cooled atom cloud to a quadrupole magnetic trap

Observation and mitigation of ion trapping in Indus-2

Spectral observations of big objects

Finite amplitude solitary structures of coupled kinetic Alfven-acoustic waves in dense plasmas

Bose gases in one-dimensional harmonic trap

Optical probing of Eu ions confined in an RF trap

Heavy flavour hadron spectroscopy: An overview

Feedback-controlled electro-kinetic traps for single-molecule spectroscopy

Similar Documents

Quantum logic gates using coherent population trapping states

Compact fibre-based coherent anti-Stokes Raman scattering spectroscopy and interferometric coherent anti-Stokes Raman scattering from a single femtosecond fibre-laser oscillator

Optimization of transfer of laser-cooled atom cloud to a quadrupole magnetic trap

Observation and mitigation of ion trapping in Indus-2

Spectral observations of big objects

Finite amplitude solitary structures of coupled kinetic Alfven-acoustic waves in dense plasmas

Bose gases in one-dimensional harmonic trap

Optical probing of Eu ions confined in an RF trap

Heavy flavour hadron spectroscopy: An overview

Feedback-controlled electro-kinetic traps for single-molecule spectroscopy