NDLI: Expression of the rgMT gene, encoding for a rice metallothionein-like protein in Saccharomyces cerevisiae and Arabidopsis thaliana

Content Provider	Springer Nature Link
Author	JIN, SHUMEI SUN, DAN WANG, JI LI, YING WANG, XINWANG LIU, SHENKUI
Copyright Year	2014
Abstract	Metallothioneins (MTs) are cysteine-rich proteins of low molecular weight with many attributed functions, such as providing protection against metal toxicity, being involved in regulation of metal ions uptake that can impact plant physiology and providing protection against oxidative stress. However, the precise function of the metallothionein-like proteins such as the one coded for rgMT gene isolated from rice (Oryza sativa L.) is not completely understood. The whole genome analysis of rice (O. sativa) showed that the rgMT gene is homologue to the Os11g47809 on chromosome 11 of O. sativa sp. japonica genome. This study used the rgMT coding sequence to create transgenic lines to investigate the subcellular localization of the protein, as well as the impact of gene expression in yeast (Saccharomyces cerevisiae) and Arabidopsis thaliana under heavy metal ion, salt and oxidative stresses. The results indicate that the rgMT gene was expressed in the cytoplasm of transgenic cells. Yeast cells transgenic for rgMT showed vigorous growth compared to the nontransgenic controls when exposed to 7 mM CuCl$_{2}$, 10 mM FeCl$_{2}$, 1 M NaCl, 24 mM NaHCO$_{3}$ and 3.2 mM H$_{2}$O$_{2}$, but there was no significant difference for other stresses tested. Similarly, Arabidopsis transgenic for rgMT displayed significantly improved seed germination rates over that of the control when the seeds were stressed with 100 μM CuCl$_{2}$ or 1 mM H$_{2}$O$_{2}$. Increased biomass was observed in the presence of 100 μM CuCl$_{2}$, 220 μM FeCl$_{2}$, 3 mM Na$_{2}$CO$_{3}$, 5 mM NaHCO$_{3}$ or 1 mM H$_{2}$O$_{2}$. These results indicate that the expression of the rice rgMT gene in transgenic yeast and Arabidopsis is implicated in improving their tolerance for certain salt and peroxide stressors.
Starting Page	709
Ending Page	718
Page Count	10
File Format	PDF
ISSN	00221333
Journal	Journal of Genetics
Volume Number	93
Issue Number	3
e-ISSN	09737731
Language	English
Publisher	Springer India
Publisher Date	2014-11-26
Publisher Institution	Indian Academy of Sciences
Publisher Place	India
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Arabidopsis metal-ion tolerance metallothionein rice salt tolerance yeast transgenic gene expression. Life Sciences Microbial Genetics and Genomics Plant Genetics & Genomics Animal Genetics and Genomics Evolutionary Biology
Content Type	Text
Resource Type	Article
Subject	Genetics

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