NDLI: Enhanced Gene Delivery Mediated by Low Molecular Weight Chitosan/DNA Complexes: Effect of pH and Serum

Content Provider	Springer Nature Link
Author	Nimesh, Surendra Thibault, Marc M. Lavertu, Marc Buschmann, Michael D.
Copyright Year	2010
Abstract	This study was designed to systematically evaluate the influence of pH and serum on the transfection process of chitosan–DNA complexes, with the objective of maximizing their efficiency. The hydrodynamic diameter of the complexes, measured by dynamic light scattering (DLS), was found to increase with salt and pH from 243 nm in water to 1244 nm in PBS at pH 7.4 and aggregation in presence of 10% serum. The cellular uptake of complexes into HEK 293 cells assessed by flow cytometry and confocal fluorescent imaging was found to increase at lower pH and serum. Based on these data, new methodology were tested and high levels of transfection (>40%) were achieved when transfection was initiated at pH 6.5 with 10% serum for 8–24 h to maximize uptake and then the media was changed to pH 7.4 with 10% serum for an additional 24–40 h period. Cytotoxicity of chitosan/DNA complexes was also considerably lower than Lipofectamine™. Our study demonstrates that the evaluation of the influence of important parameters in the methodology of transfection enables the understanding of crucial physicochemical and biological mechanisms which allows for the design of methodologies maximising transgene expression.
Starting Page	182
Ending Page	196
Page Count	15
File Format	PDF
ISSN	10736085
Journal	Molecular Biotechnology
Volume Number	46
Issue Number	2
e-ISSN	15590305
Language	English
Publisher	Humana Press Inc
Publisher Date	2010-05-08
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chitosan Complexes Uptake Transfection Cytotoxicity Human Genetics Biological Techniques Protein Science Cell Biology Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Enhanced Gene Delivery Mediated by Low Molecular Weight Chitosan/DNA Complexes: Effect of pH and Serum

Improved stability and efficacy of chitosan/pDNA complexes for gene delivery

Structure Dependence of Lysosomal Transit of Chitosan-Based Polyplexes for Gene Delivery

N-Succinyl-chitosan grafted with low molecular weight polyethylenimine as a serum-resistant gene vector

Chitosan-mediated non-viral gene delivery with improved serum stability and reduced cytotoxicity

Cationic β-Cyclodextrin–Chitosan Conjugates as Potential Carrier for pmCherry-C1 Gene Delivery

Chitosan enhanced gene delivery of cationic liposome via non-covalent conjugation

The Kinetics of Polyethylenimine-Mediated Transfection in Suspension Cultures of Chinese Hamster Ovary Cells

Application of Chitosan-Based Nanocarriers in Tumor-Targeted Drug Delivery

Enhanced Gene Delivery Mediated by Low Molecular Weight Chitosan/DNA Complexes: Effect of pH and Serum

Similar Documents

Enhanced Gene Delivery Mediated by Low Molecular Weight Chitosan/DNA Complexes: Effect of pH and Serum

Improved stability and efficacy of chitosan/pDNA complexes for gene delivery

Structure Dependence of Lysosomal Transit of Chitosan-Based Polyplexes for Gene Delivery

N-Succinyl-chitosan grafted with low molecular weight polyethylenimine as a serum-resistant gene vector

Chitosan-mediated non-viral gene delivery with improved serum stability and reduced cytotoxicity

Cationic β-Cyclodextrin–Chitosan Conjugates as Potential Carrier for pmCherry-C1 Gene Delivery

Chitosan enhanced gene delivery of cationic liposome via non-covalent conjugation

The Kinetics of Polyethylenimine-Mediated Transfection in Suspension Cultures of Chinese Hamster Ovary Cells

Application of Chitosan-Based Nanocarriers in Tumor-Targeted Drug Delivery

Enhanced Gene Delivery Mediated by Low Molecular Weight Chitosan/DNA Complexes: Effect of pH and Serum