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| Content Provider | Springer Nature Link |
|---|---|
| Author | Wang, Huixian Hays, John B. |
| Copyright Year | 2000 |
| Abstract | Analyses in vitro of correction of DNA mismatches have been pivotal in biochemical dissection of mismatch repair pathways. However, the complex procedures needed to prepare DNA substrates for mismatch repair have posed substantial barriers to investigators who wish to pursue such analyses. Here we describe a simple, efficient way to prepare a variety of mismatched DNA substrates. We use in our procedure high-copy-number pUC19-derived plasmids, and a newly commercially available endonuclease N.BstNBI that makes site-specific single-strand nicks. The ability to prepare large substrate quantities in a relatively short time and to construct wider ranges of different mismatches in various sequence contexts will facilitate future research. |
| Starting Page | 97 |
| Ending Page | 104 |
| Page Count | 8 |
| File Format | |
| ISSN | 10736085 |
| Journal | Molecular Biotechnology |
| Volume Number | 15 |
| Issue Number | 2 |
| e-ISSN | 15590305 |
| Language | English |
| Publisher | Humana Press |
| Publisher Date | 2000-01-01 |
| Publisher Place | Totowa |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Neurosciences Neurology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Molecular Biology Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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