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| Content Provider | Springer Nature Link |
|---|---|
| Author | Duraisamy, Raghu Natesan, Senthil Muthurajan, Raveendran Gandhi, Karthikayan Lakshmanan, Pugalendhi Karuppusamy, Nageswari Chokkappan, Mohan |
| Copyright Year | 2012 |
| Abstract | Cassava mosaic disease, caused by cassava mosaic geminiviruses are transmitted by Bemisia tabaci. The B. tabaci adults from colonies reared on virus free cassava plant produced from apical meristem culture was studied to determine their ability to transmit Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV) from cassava to cassava. Virus free plants were confirmed by polymerase chain reaction (PCR) using geminivirus degenerate primers. The virus acquisition access period (AAP) of 48 h on virus infected cassava leaves and 48 h virus inoculation access periods on virus free healthy leaves were investigated. Both ICMV and SLCMV were absolutely transmitted by whiteflies reared on cassava. Virus specific primers were designed in the replicase region and used to detect virus in B. tabaci after different AAP. The PCR amplified replicase genes from virus transmitted cassava leaves were cloned the plasmid DNA was isolated from a recombinant colony of E. coli DH5α after their confirmation by colony PCR and sequenced them. The nucleotide sequences obtained from automated DNA sequencing were confirmed as ICMV and SLCMV replicase gene after homology searching by BLAST and found to be a new isolates. The nucleotide sequences of new isolates were submitted in GenBank (accession number JN652126 and JN595785). |
| Starting Page | 150 |
| Ending Page | 158 |
| Page Count | 9 |
| File Format | |
| ISSN | 10736085 |
| Journal | Molecular Biotechnology |
| Volume Number | 53 |
| Issue Number | 2 |
| e-ISSN | 15590305 |
| Language | English |
| Publisher | Humana Press Inc |
| Publisher Date | 2012-02-03 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | B. tabaci Cloning Meristem culture Sequencing Virus transmission Biotechnology Biochemistry Cell Biology Protein Science Biological Techniques Human Genetics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Molecular Biology Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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