NDLI: Large-scale extraction of proteins

Content Provider	Springer Nature Link
Author	Cunha, Teresa Aires Barros, Raquel
Copyright Year	2002
Abstract	The production of foreign proteins using selected host with the necessary posttranslational modifications is one of the key successes in modern biotechnology. This methodology allows the industrial production of proteins that otherwise are produced in small quantities. However, the separation and purification of these proteins from the fermentation media constitutes a major bottleneck for the widespread commercialization of recombinant proteins. The major production costs (50–90%) for typical biological product resides in the purification strategy. There is a need for efficient, effective, and economic large-scale bioseparation techniques, to achieve high purity and high recovery, while maintaining the biological activity of the molecule.Aqueous two-phase systems (ATPS) allow process integration as simultaneously separation and concentration of the target protein is achieved, with posterior removal and recycle of the polymer. The ease of scaleup combined with the high partition coefficients obtained allow its potential application in large-scale downstream processing of proteins produced by fermentation.The equipment and the methodology for aqueous two-phase extraction of proteins on a large scale using mixer-settler and column contractors are described. The operation of the columns, either stagewise or differential, are summarized. A brief description of the methods used to account for mass transfer coefficients, hydrodynamics parameters of hold-up, drop size, and velocity, back mixing in the phases, and flooding performance, required for column design, is also provided.
Starting Page	29
Ending Page	40
Page Count	12
File Format	PDF
ISSN	10736085
Journal	Molecular Biotechnology
Volume Number	20
Issue Number	1
e-ISSN	15590305
Language	English
Publisher	Humana Press
Publisher Date	2002-01-01
Publisher Place	Totowa
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Neurosciences Neurology
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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