NDLI: Naturally occurring catalytic antibodies: evidence for preferred development of the catalytic function in IgA class antibodies

Content Provider	Springer Nature Link
Author	Mitsuda, Yukie Planque, Stephanie Hara, Mariko Kyle, Robert Taguchi, Hiroaki Nishiyama, Yasuhiro Paul, Sudhir
Copyright Year	2007
Abstract	IgG class antibodies express catalytic activities rarely and at very low levels. Here, we studied polyclonal IgA and IgG preparations from healthy human sera and saliva for the ability to hydrolyze model peptidyl-aminomethylcoumarin (peptide-AMC) substrates. These substrates permit objective evaluation of the catalytic potential of the antibody classes with minimal effects of noncovalent interactions occurring at sites remote from the reaction center. The IgA preparations hydrolyzed Glu-Ala-Arg-AMC at rates 3-orders of magnitude greater than IgG preparations from the same individuals. The cleavage occurred preferentially on the C terminal side of a basic residue. The activity was confirmed using monoclonal IgAs isolated from patients with multiple myeloma. Active site-directed inhibitors of serine proteases inhibited the catalytic activity and were bound irreversibly by the IgA, suggesting the involvement of a serine protease-like mechanism similar to that utilized by previously described IgM antibodies. These observations suggest that mechanisms underlying B cell clonal selection favor the retention and improvement of catalytic activity in the IgA, but not the IgG compartment of the immune response.
Starting Page	113
Ending Page	122
Page Count	10
File Format	PDF
ISSN	10736085
Journal	Molecular Biotechnology
Volume Number	36
Issue Number	2
e-ISSN	15590305
Language	English
Publisher	Humana Press Inc
Publisher Date	2007-04-25
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Catalytic antibody IgA Mucosal immunity Serine protease Phosphonate ester Neurology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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