NDLI: Recombinant HAP Phytase of the Thermophilic Mold Sporotrichum thermophile: Expression of the Codon-Optimized Phytase Gene in Pichia pastoris and Applications

Content Provider	Springer Nature Link
Author	Ranjan, Bibhuti Satyanarayana, T.
Copyright Year	2016
Abstract	The codon-optimized phytase gene of the thermophilic mold Sporotrichum thermophile (St-Phy) was expressed in Pichia pastoris. The recombinant P. pastoris harboring the phytase gene (rSt-Phy) yielded a high titer of extracellular phytase (480 ± 23 U/mL) on induction with methanol. The recombinant phytase production was ~40-fold higher than that of the native fungal strain. The purified recombinant phytase (rSt-Phy) has the molecular mass of 70 kDa on SDS-PAGE, with K $_{m}$ and V $_{max}$ (calcium phytate), k $_{cat}$ and k $_{cat}$/K $_{m}$ values of 0.147 mM and 183 nmol/mg s, 1.3 × 10$^{3}$/s and 8.84 × 10$^{6}$/M s, respectively. Mg$^{2+}$ and Ba$^{2+}$ display a slight stimulatory effect, while other cations tested exert inhibitory action on phytase. The enzyme is inhibited by chaotropic agents (guanidinium hydrochloride, potassium iodide, and urea), Woodward’s reagent K and 2,3-bunatedione, but resistant to both pepsin and trypsin. The rSt-Phy is useful in the dephytinization of broiler feeds efficiently in simulated gut conditions of chick leading to the liberation of soluble inorganic phosphate with concomitant mitigation in antinutrient effects of phytates. The addition of vanadate makes it a potential candidate for generating haloperoxidase, which has several applications.
Starting Page	137
Ending Page	147
Page Count	11
File Format	PDF
ISSN	10736085
Journal	Molecular Biotechnology
Volume Number	58
Issue Number	2
e-ISSN	15590305
Language	English
Publisher	Springer US
Publisher Date	2016-01-12
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Thermophilic mold Pichia pastoris Recombinant phytase Broiler feeds Dephytinization Haloperoxidase Biotechnology Biochemistry Cell Biology Protein Science Biological Techniques Human Genetics
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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