NDLI: Novel Interactive Partners of Neuroligin 3: New Aspects for Pathogenesis of Autism

Content Provider	Springer Nature Link
Author	Shen, Chen Huo, Li rong Zhao, Xin liang Wang, Pei rong Zhong, Nanbert
Copyright Year	2014
Abstract	Autism is a neurodevelopmental disorder with a strong genetic predisposition. Neurolign 3 (NLGN3) as a postsynaptic transmembrane protein, functions in both neuron synaptogenesis and glia-neuron communications. Previously, a gain of function mutation (R451C) in NLGN3 was identified in autistic patients, which illustrates the involvement of NLGN3 in autism pathogenesis. As proper synaptic targeting and functioning are controlled by intracellular protein interactions, in the current study, we tried to discover the intracellular regulation network in which NLGN3 might be involved by a yeast two-hybrid-based interactor identification. Fifty-one protein candidate partners were identified after screening a human fetal complementary DNA (cDNA) library with an intracellular fragment of NLGN3. The interactions of NLGN3 with a subset of candidates, including EEF1A1, FLNA, ITPRIP, CYP11A1, MT-CO2, GPR175, ACOT2, and QPRT, were further validated in human neuroblastoma cells or brain tissues. Furthermore, our study suggested that NLGN3 was functioning in cytosolic calcium balance and participating in calcium-regulated cellular processes. Our findings of novel NLGN3 binding partners provide evidences of involvement of NLGN3 in multiple biological pathways, especially calcium regulating and mitochondrial function, thus suggesting further significance. This new data not only leads to a better understanding of the physiological functions of NLGN3, but also provide new aspects for pathogenesis of autism.
Starting Page	89
Ending Page	101
Page Count	13
File Format	PDF
ISSN	08958696
Journal	Journal of Molecular Neuroscience
Volume Number	56
Issue Number	1
e-ISSN	15591166
Language	English
Publisher	Springer US
Publisher Date	2014-12-03
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	NLGN3 Yeast two-hybrid Co-immunoprecipitation Protein-protein interaction Co-localization Cytosolic calcium Neurosciences Neurochemistry Cell Biology Proteomics Neurology
Content Type	Text
Resource Type	Article
Subject	Medicine Cellular and Molecular Neuroscience

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