NDLI: Neuroprotective Effects of Paeoniflorin, But Not the Isomer Albiflorin, are Associated with the Suppression of Intracellular Calcium and Calcium/Calmodulin Protein Kinase II in PC12 Cells

Content Provider	Springer Nature Link
Author	Wang, Di Tan, Qing Rong Zhang, Zhang Jin
Copyright Year	2013
Abstract	The root of Paeonia lactiflora Pall (family Ranunculaceae) or peony root, a herbal medicine, possesses therapeutic potential for neurodegenerative diseases. The isomers paeoniflorin (PF) and albiflorin (AF) are major constituents contained in peony root. Our previous study has shown notable neuroprotective effects of PF. In the present study, we further compared the effects of AF and PF against glutamate (Glu)-induced cell damage and the underlying mechanisms in differentiated PC12 cells. Both AF and PF significantly ameliorated Glu-induced reduction of cell viability, nuclear and mitochondrial apoptotic alteration, reactive oxygen species accumulation, and B-cell lymphoma 2 (Bcl-2)/Bax ratio. The two isomers also enhanced phosphorylation of AKT and its downstream element glycogen synthase kinase-3β, and this effect was abrogated by the AKT inhibitor LY294002. PF, but not AF, however, suppressed intracellular Ca$^{2+}$ overload and the expression of calcium/calmodulin protein kinase II (CaMKII). The improvement of cell damage by the CaMKII inhibitor KN93 further confirms the role of CaMKII in PF-mediated neuroprotection. These results suggest that both AF and PF possess robust effects in protecting neuronal cells against Glu toxicity. PF further displayed remarkable effects in preventing intracellular Ca$^{2+}$ overload and suppressing overexpression of CaMKII. Differential mechanisms may be involved in neuroprotective action of the two isomers.
Starting Page	581
Ending Page	590
Page Count	10
File Format	PDF
ISSN	08958696
Journal	Journal of Molecular Neuroscience
Volume Number	51
Issue Number	2
e-ISSN	15591166
Language	English
Publisher	Springer US
Publisher Date	2013-05-22
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Paeoniflorin Albiflorin Glutamate Apoptosis AKT CaMKII Neurosciences Neurochemistry Cell Biology Proteomics Neurology
Content Type	Text
Resource Type	Article
Subject	Medicine Cellular and Molecular Neuroscience

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