NDLI: Oxygen Tension Modulates Neurite Outgrowth in PC12 Cells Through A Mechanism Involving HIF and VEGF

Content Provider	Springer Nature Link
Author	Genetos, Damian C. Cheung, Whitney K. Decaris, Martin L. Leach, J. Kent
Copyright Year	2010
Abstract	Cell-based approaches are a promising therapeutic strategy for treating injuries to the nervous system, but the optimal means to promote neurite extension and direct cellular behavior are unclear. Previous studies have examined the behavior of neural-like cells in ambient air (21% oxygen tension), yet these conditions are not representative of the physiological oxygen microenvironment of neural tissues. We hypothesized that neuronal differentiation of a model neural cell line (PC12) could be controlled by modulating local oxygen tension. Compared to ambient conditions, PC12 cells cultured in reduced oxygen exhibited significant increases in neurite extension and total neurite length, with 4% oxygen yielding the highest levels of both indicators. We confirmed neurite extension was mediated through oxygen-responsive mechanisms using small molecules that promote or inhibit HIF-1α stabilization. The hypoxic target gene Vegf was implicated as a neurotrophic factor, as neurite formation at 21% oxygen was mimicked with exogenous VEGF, and a VEGF-neutralizing antibody attenuated neurite formation under reduced oxygen conditions. These findings demonstrate that behavior of neural-like cells is driven by the oxygen microenvironment via VEGF function, and suggest promising approaches for future applications in neural repair.
Starting Page	360
Ending Page	366
Page Count	7
File Format	PDF
ISSN	08958696
Journal	Journal of Molecular Neuroscience
Volume Number	40
Issue Number	3
e-ISSN	15591166
Language	English
Publisher	Humana Press Inc
Publisher Date	2010-01-27
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Oxygen tension Neurite extension PC12 Hypoxia-inducible factor Vascular endothelial growth factor Neurology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Medicine Cellular and Molecular Neuroscience

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Oxygen tension modulates neurite outgrowth in PC12 cells through a mechanism involving HIF and VEGF.

Oxygen Tension Modulates Neurite Outgrowth in PC12 Cells Through A Mechanism Involving HIF and VEGF

Oxygen tension modulates neurite outgrowth in pc12 cells through a mechanism involving hif and vegf (2009).

Antibodies Directed to Neisseria gonorrhoeae Impair Nerve Growth Factor-Dependent Neurite Outgrowth in Rat PC12 Cells

Transcription Factor Brn-3b Overexpression Enhances Neurite Outgrowth in PC12 Cells Under Condition of Hypoxia

Endoplasmic Reticulum (ER) Stress and Hypoxia Response Pathways Interact to Potentiate Hypoxia-inducible Factor 1 (HIF-1) Transcriptional Activity on Targets Like Vascular Endothelial Growth Factor (VEGF)

Activation of STAT3 by Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) During PACAP-Promoted Neurite Outgrowth of PC12 Cells

Enhanced Hypoxia-Inducible Factor (HIF)-1α Stability Induced by 5-Hydroxymethyl-2-Furfural (5-HMF) Contributes to Protection against Hypoxia

Nerve growth factor induces neurite outgrowth of PC12 cells by promoting Gβγ-microtubule interaction

Oxygen Tension Modulates Neurite Outgrowth in PC12 Cells Through A Mechanism Involving HIF and VEGF

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Oxygen tension modulates neurite outgrowth in PC12 cells through a mechanism involving HIF and VEGF.

Oxygen Tension Modulates Neurite Outgrowth in PC12 Cells Through A Mechanism Involving HIF and VEGF

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Oxygen Tension Modulates Neurite Outgrowth in PC12 Cells Through A Mechanism Involving HIF and VEGF