NDLI: Neuroprotective Effect of Protease-Activated Receptor-2 in the Hypoxia-Induced Apoptosis of Rat RGC-5 Cells

Content Provider	Springer Nature Link
Author	Peng, Yanli Zhang, Jiaping Xu, Haiwei He, Jianrong Ying, Xi Wang, Yi
Copyright Year	2012
Abstract	Hypoxia-induced apoptosis of retinal ganglion cells (RGCs) is regarded as a pivotal pathological process in various ocular diseases. Protease-activated receptor-2 (PAR-2) is involved in the regulation of cell inflammation, differentiation, and apoptosis in many cell types and tissues, but the role of PAR-2 in RGCs under pathological conditions remains unknown. The purpose of this study was to investigate the role of PAR-2 in the apoptosis of RGCs under hypoxic stress. An immortalized rat RGC line (RGC-5) was exposed to hypoxia (5 % O$_{2}$). The expression and location of PAR-2 in RGC-5 cells under hypoxia stress were investigated using real-time PCR, western blotting and immunocytochemistry. Cell viability was determined using the Cell Counting Kit-8 assay. Apoptosis was detected using Hoechst 33342 staining and AnnexinV-FITC/PI assays. The role of Bcl-2, Bax, and the active subunit of caspase-3 was also investigated. The results showed that PAR-2 was functionally expressed in RGC-5 cells and up-regulated at both mRNA and protein levels under hypoxic stress. The PAR-2 selective agonist, SLIGRL, rescued RGC-5 cells from hypoxia-induced apoptosis through up-regulation of the Bcl-2/Bax ratio and down-regulation of caspase-3 activation. This study provides the first evidence that PAR-2 has a protective effect against the hypoxia-induced apoptosis of RGC-5 cells.
Starting Page	98
Ending Page	108
Page Count	11
File Format	PDF
ISSN	08958696
Journal	Journal of Molecular Neuroscience
Volume Number	50
Issue Number	1
e-ISSN	15591166
Language	English
Publisher	Humana Press Inc
Publisher Date	2012-09-05
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Protease-activated receptor-2 RGC-5 cells Hypoxia Apoptosis Caspase-3 Bcl-2/Bax ratio Neurosciences Neurochemistry Cell Biology Proteomics Neurology
Content Type	Text
Resource Type	Article
Subject	Medicine Cellular and Molecular Neuroscience

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