NDLI: Characterization of the expression of key adenoviral receptors CAR and integrin β$_{3}$/β$_{5}$ subunits on the membrane of human NT2 neurons

Content Provider	Springer Nature Link
Author	Huang, Deqi Desbois, Angele Chen, Gao Fang, Hung Hou, Sheng T.
Copyright Year	2004
Abstract	Expression of therapeutic gene products in differentiated human NT2 neurons (NT2/Ns) is being explored for ex vivo gene therapy of human neurological diseases. In this study we determined the efficiency of adenovirus (Ad)-mediated gene delivery into NT2/Ns and characterized the expression of several key receptors known to be required for efficient Ad-mediated gene delivery. Undifferentiated NT2 cells and NT2/Ns were infected by Ad expressing green fluorescent protein at an efficiency of 33% and 17%, respectively—percentages much lower than the 92% infectivity obtained from a human non-neuronal cell line A549 cells. This relatively low infectivity of NT2/Ns might be caused by the extremely low expression of integrin subunit β$_{3}$ and the reduced expression of β$_{5}$ during differentiation. The expression of coxsackie-Ad receptor (CAR) was relatively high and remained constant during differentiation. Blocking CAR receptor using an antibody specific against CAR reduced Ad infectivity in a dose-dependent manner. These observations suggest that modulating the expression of integrin subunits β$_{3/5}$ or the functional heterodimer α$_{v}$β$_{3/5}$ in human NT2/Ns may enhance adenoviral infectivity of NT2/Ns.
Starting Page	323
Ending Page	328
Page Count	6
File Format	PDF
ISSN	08958696
Journal	Journal of Molecular Neuroscience
Volume Number	24
Issue Number	2
e-ISSN	15591166
Language	English
Publisher	Humana Press
Publisher Date	2004-01-01
Publisher Place	Totowa
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Neurosciences Neurology
Content Type	Text
Resource Type	Article
Subject	Medicine Cellular and Molecular Neuroscience

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