NDLI: Effects of PACAP and VIP on cAMP-generating System and Proliferation of C6 Glioma Cells

Content Provider	Springer Nature Link
Author	Sokolowska, Paulina wak, Jerzy Z.
Copyright Year	2008
Abstract	An identification of PAC$_{1}$- and VPAC-type receptors in a great number of neoplastic cells gave rise to intensive studies on the biochemical and physiological role of the mentioned peptides in cancers. Our earlier studies focused on effects of pituitary adenylate cyclase-activating peptide (PACAP) and vasoactive intestinal peptide (VIP) in C6 glioma cells have shown their stimulatory receptor-mediated action on the cyclic adenosine monophosphate (cAMP)-generating system. In the present study, we demonstrated that truncated peptides, i.e., PACAP6-38 and VIP6-28, both produced a significant inhibition of the VIP-induced increase in cAMP production, whereas only PACAP6-38 did antagonize the PACAP-38 effect. In contrast to the well-expressed PACAP-38 and VIP effects on cAMP production in C6 cells, helodermin and secretin were poorly active as cAMP stimulators in this cell line, displaying some activity only at a high 5-μM dose. PACAP-38 and, to a lesser extent VIP stimulated the proliferation of C6 glioma cells, which was shown by an increased incorporation of $^{3}$H-thymidine into the cells, and the effects of these two peptides were antagonized by PACAP6-38. The truncated PACAP (10 μM) by itself significantly inhibited C6 cell proliferation. The study with the use of forskolin and dibutyryl-cAMP revealed that the growth effects of PACAP were cAMP independent. Our findings suggest that glioma C6 cells possess PAC$_{1}$- and VPAC-type receptors, but the density of PAC$_{1}$ seems to be much larger than VPAC receptors. Although the proliferative activity of PACAP and VIP is mediated via the PAC$_{1}$-type receptor, the signaling cascade underlying this phenomenon does not seem to involve cAMP.
Starting Page	286
Ending Page	291
Page Count	6
File Format	PDF
ISSN	08958696
Journal	Journal of Molecular Neuroscience
Volume Number	36
Issue Number	1-3
e-ISSN	15591166
Language	English
Publisher	Humana Press Inc
Publisher Date	2008-05-20
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	PACAP VIP cAMP Proliferation Rat glioma cells Neurology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Medicine Cellular and Molecular Neuroscience

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