NDLI: Differences in expression of GABA$_{A}$ receptor subunits, but not benzodiazepine binding, in the chick brainstem auditory system

Content Provider	Springer Nature Link
Author	Hyson, Richard L. Sadler, Karen A.
Copyright Year	1997
Abstract	Neurons in nucleus magnocellularis (NM) and nucleus laminaris (NL) of the chick brainstem auditory system show an unusual physiological response to GABA. Examination of these nuclei usingin situ hybridization for GABA$_{A}$ receptor subunits showed a differential expression of the γ$_{2}$ and α$_{1}$ subunits. The γ$_{2}$ subunit was found in both NM and NL, but the α$_{1}$ subunit was found in NL only. Like NL, other areas of the tissue section that showed labeling with the γ$_{2}$ probe, such as the medial vestibular nucleus (VeM) and granule cells of the cerebellum (CB), also labeled with the α$_{1}$ probe. Thus, given that NM labeled with the γ$_{2}$ probe, the absence of the α$_{1}$ subunit was unusual in this tissue. This difference in subunit composition suggests that there may also be a difference in GABA receptor function in NM compared to these neighboring areas. One feature of the GABA$_{A}$ receptor believed to be related to the presence of γ$_{2}$ and α$_{1}$ subunits is specific pharmacological properties of the benzodiazepine modulatory site. It has been proposed that the α$_{1}$ subunit is necessary for producing a GABA$_{A}$ receptor with a benzodiazepine site that has Type I binding characteristics. The present experiments challenge this hypothesis. Based on the differential presence of the α$_{1}$ subunit, one would expect that GABA receptors in NM would show different benzodiazepine binding properties than NL, VeM, and CB. However, displacement of$^{3}$H-flunitrazepam binding using CL 218,872, which differentiates between the Type I and Type II receptors, showed no difference between these areas. Additionally, the relatively high affinity for CL 218,872 suggests that even NM contains Type I receptors.
Starting Page	193
Ending Page	205
Page Count	13
File Format	PDF
ISSN	08958696
Journal	Journal of Molecular Neuroscience
Volume Number	8
Issue Number	3
e-ISSN	15591166
Language	English
Publisher	Humana Press
Publisher Date	1997-01-01
Publisher Place	Totowa
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Neurosciences Neurology
Content Type	Text
Resource Type	Article
Subject	Medicine Cellular and Molecular Neuroscience

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