NDLI: Corpus Callosum: a Favorable Target for rSFV-Mediated Gene Transfer to Rat Brain with Broad and Efficient Expression

Content Provider	Springer Nature Link
Author	Li, Zhao Jian Sun, Peng Zhang, Hong Di Li, Shi Fang Liu, Xia Wang, Ren Zhi
Copyright Year	2010
Abstract	Recombinant Semliki Forest virus (rSFV), as a new kind of neurotropic vector system, has great potential of gene therapy for stroke. However, very little is known about its transduction characteristics in cerebral cortex or corpus callosum (CC) in vivo, which are common targets for gene transfer in experimental stroke therapy. Here, we investigate and compare rSFV-mediated gene expression at above two brain regions in rat; 2.0 × 10$^{7}$ IU of rSFV encoding green fluorescent protein (rSFV-GFP) was locally injected into CC or cerebral cortex in two groups. At 36 h following injection, the number of GFP-positive cells, GFP distribution volume, and GFP expression level were examined in the rat brain of each group using continuous frozen sections and enzyme-linked immunosorbent assay. rSFV vector displayed noticeably different transduction patterns in CC and cerebral cortex in vivo. CC injection of vector increased GFP-positive cell number by 802%, GFP transduction volume by 958%, and GFP expression level by 508% compared with cortical injection (all P < 0.01). We concluded that rSFV CC delivery significantly enhances transduction efficiency in rat brain with its ability to achieve transgene extensive transduction and abundant expression, and CC may be a favorable target for improving rSFV-based gene delivery efficiency to brain.
Starting Page	255
Ending Page	260
Page Count	6
File Format	PDF
ISSN	08958696
Journal	Journal of Molecular Neuroscience
Volume Number	42
Issue Number	2
e-ISSN	15591166
Language	English
Publisher	Humana Press Inc
Publisher Date	2010-05-12
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Brain ischemia Cerebral cortex Corpus callosum Gene transfer Green fluorescent protein Semliki Forest virus Neurology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Medicine Cellular and Molecular Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Neurons and oligodendrocytes in the mouse brain differ in their ability to replicate Semliki Forest virus

A novel neurotropic expression vector based on the avirulent A7(74) strain of Semliki Forest virus

Minocycline attenuation of rat corpus callosum abnormality mediated by low-dose lipopolysaccharide-induced microglia activation

Pseudo-typed Semliki Forest virus delivers EGFP into neurons

Transcriptome analysis of amoeboid and ramified microglia isolated from the corpus callosum of rat brain

Gene Expression Profiling of Rat Cerebral Cortex Development Using cDNA Microarrays

Neuroepithelial denudation in the hyh mutant mice with congenital hydrocephalus produces agenesis of corpus callosum and alteration in the cerebral cortex

Early suppressive antiretroviral therapy in HIV infection is associated with measurable changes in the corpus callosum

Activation of cPLA$_{2}$, PKC, and ERKs in the Rat Cerebral Cortex During Ischemia/Reperfusion

Corpus Callosum: a Favorable Target for rSFV-Mediated Gene Transfer to Rat Brain with Broad and Efficient Expression

Similar Documents

Neurons and oligodendrocytes in the mouse brain differ in their ability to replicate Semliki Forest virus

A novel neurotropic expression vector based on the avirulent A7(74) strain of Semliki Forest virus

Minocycline attenuation of rat corpus callosum abnormality mediated by low-dose lipopolysaccharide-induced microglia activation

Pseudo-typed Semliki Forest virus delivers EGFP into neurons

Transcriptome analysis of amoeboid and ramified microglia isolated from the corpus callosum of rat brain

Gene Expression Profiling of Rat Cerebral Cortex Development Using cDNA Microarrays

Neuroepithelial denudation in the hyh mutant mice with congenital hydrocephalus produces agenesis of corpus callosum and alteration in the cerebral cortex

Early suppressive antiretroviral therapy in HIV infection is associated with measurable changes in the corpus callosum

Activation of cPLA$_{2}$, PKC, and ERKs in the Rat Cerebral Cortex During Ischemia/Reperfusion

Corpus Callosum: a Favorable Target for rSFV-Mediated Gene Transfer to Rat Brain with Broad and Efficient Expression