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| Content Provider | Springer Nature Link |
|---|---|
| Author | Padua, Francesco Marrelli, Massimo Palmieri, Francesca Tatullo, Marco |
| Copyright Year | 2016 |
| Abstract | Recent studies have identified a new human dental derived progenitor cell population with multi-lineage differentiation potential referred to as human periapical cyst mesenchymal stem cells (hPCy-MSCs). In the present study, we compared two subpopulations of hPCy-MSCs characterised by the low or high expression of CD146 to establish whether this expression can regulate their stem cell properties. Using flow cytometry, we evaluated the stem cell marker profile of hPCy-MSCs during passaging. Furthermore, CD146$^{Low}$ and CD146$^{High}$ cells were sorted by magnetic beads and subsequently both cell populations were evaluated for differences in their proliferation, self-renewal, stem cell surface markers, stemness genes expression and osteogenic differentiation potential.We found that hPCy-MSCs possessed a stable expression of several mesenchymal stem cell surface markers, whereas CD146 expression declined during passaging.In addition, sorted CD146$^{Low}$ cells proliferated significantly faster, displayed higher colony-forming unit-fibroblast capacity and showed higher expression of Klf4 when compared to the CD146$^{High}$ subset. Significantly, the osteogenic potential of hPCy-MSCs was greater in the CD146$^{Low}$ than in CD146$^{High}$ population. These results demonstrate that CD146 is spontaneously downregulated with passaging at both mRNA and protein levels and that the high expression of CD146 reduces the proliferative, self-renewal and osteogenic differentiation potential of hPCy-MSCs. In conclusion, our study demonstrates that changes in the expression of CD146 can influence the stem cell properties of hPCy-MSCs. |
| Starting Page | 592 |
| Ending Page | 603 |
| Page Count | 12 |
| File Format | |
| ISSN | 15508943 |
| Journal | Stem Cell Reviews and Reports |
| Volume Number | 12 |
| Issue Number | 5 |
| e-ISSN | 15586804 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2016-07-12 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Dental stem cells (DSCs) Human periapical cyst mesenchymal stem cells (hPCy-MSCs) CD146 MSC surface markers Magnetic cell sorting Stemness genes Osteogenic differentiation Stem Cells Regenerative Medicine/Tissue Engineering Cell Biology Biomedical Engineering |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine |
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