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| Content Provider | Springer Nature Link |
|---|---|
| Author | Oh, Bea Jun Oh, Seung Hoon Choi, Jin Myung Jin, Sang Man Shim, Woo Young Lee, Myung Shik Lee, Moon Kyu Kim, Kwang Won Kim, Jae Hyeon |
| Copyright Year | 2014 |
| Abstract | Islet transplantation has been hampered by the shortage of islet donors available for diabetes therapy. However, pluripotent stem cells (PSCs) can be an alternative source of insulin-producing cells (IPCs) because of their capacity for self-renewal and differentiation. We described a method to efficiently differentiate PSCs into IPCs by co-culturing mature islets with directed-differentiated pancreatic endoderm (PE) cells from mouse and human PSCs. PE cells co-cultured with islet cells or islet cell-derived conditioned medium (CM) showed increased expression levels of β-cell markers; significantly higher levels of proinsulin- and Newport Green (NG)-positive cells, which revealed the characteristics of insulin producing cells; and increased insulin secretion upon glucose stimulation. Co-culturing human PE cells with islet cells was also effective to differentiate PE cells into IPCs. Diabetic nude mice transplanted with co-cultured cells exhibited restored euglycemia, human C-peptide release, and improved glucose tolerance. Immunohistochemistry revealed that insulin+/C-peptide + cells existed in the grafted tissues. These results suggest that mature islet cells can increase the differentiation efficiency of PE cells into mature IPCs via paracrine effects. |
| Starting Page | 62 |
| Ending Page | 74 |
| Page Count | 13 |
| File Format | |
| ISSN | 15508943 |
| Journal | Stem Cell Reviews and Reports |
| Volume Number | 11 |
| Issue Number | 1 |
| e-ISSN | 15586804 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2014-08-31 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Pluripotent stem cells Insulin-producing cells Co-culture β-cell differentiation Diabetes Transplantation Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine |
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