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| Content Provider | Springer Nature Link |
|---|---|
| Author | Belair, David G. Whisler, Jordan A. Valdez, Jorge Velazquez, Jeremy Molenda, James A. Vickerman, Vernella Lewis, Rachel Daigh, Christine Hansen, Tyler D. Mann, David A. Thomson, James A. Griffith, Linda G. Kamm, Roger D. Schwartz, Michael P. Murphy, William L. |
| Copyright Year | 2014 |
| Abstract | Here we describe a strategy to model blood vessel development using a well-defined induced pluripotent stem cell-derived endothelial cell type (iPSC-EC) cultured within engineered platforms that mimic the 3D microenvironment. The iPSC-ECs used here were first characterized by expression of endothelial markers and functional properties that included VEGF responsiveness, TNF-α-induced upregulation of cell adhesion molecules (MCAM/CD146; ICAM1/CD54), thrombin-dependent barrier function, shear stress-induced alignment, and 2D and 3D capillary-like network formation in Matrigel. The iPSC-ECs also formed 3D vascular networks in a variety of engineering contexts, yielded perfusable, interconnected lumen when co-cultured with primary human fibroblasts, and aligned with flow in microfluidics devices. iPSC-EC function during tubule network formation, barrier formation, and sprouting was consistent with that of primary ECs, and the results suggest a VEGF-independent mechanism for sprouting, which is relevant to therapeutic anti-angiogenesis strategies. Our combined results demonstrate the feasibility of using a well-defined, stable source of iPSC-ECs to model blood vessel formation within a variety of contexts using standard in vitro formats. |
| Starting Page | 511 |
| Ending Page | 525 |
| Page Count | 15 |
| File Format | |
| ISSN | 15508943 |
| Journal | Stem Cell Reviews and Reports |
| Volume Number | 11 |
| Issue Number | 3 |
| e-ISSN | 15586804 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2014-09-05 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Stem cell Induced pluripotent stem cell Endothelial cell Angiogenesis Vascular model Vascular function Tubulogenesis Migration Sprouting Barrier function Stem Cells Regenerative Medicine/Tissue Engineering Cell Biology Biomedical Engineering |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine |
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