NDLI: Antitumor Effect of TRAIL on Oral Squamous Cell Carcinoma using Magnetic Nanoparticle-Mediated Gene Expression

Content Provider	Springer Nature Link
Author	Miao, Leiying Liu, Chao Ge, Jiuyu Yang, Weidong Liu, Jinzhong Sun, Weibin Yang, Bai Zheng, Changyu Sun, Hongchen Hu, Qingang
Copyright Year	2014
Abstract	We developed a new magnetic nanovector to improve the efficiency and targeting of transgene therapy for oral squamous cell carcinoma (OSCC). Positively charged polymer PEI-modified Fe$_{3}$O$_{4}$ magnetic nanoparticles were tested as gene transfer vectors in the presence of a magnetic field. The Fe$_{3}$O$_{4}$ nanoparticles were prepared by a co-precipitation method and had good dispersibility in water. These nanoparticles modified by PEI were combined with negatively charged pACTERT-EGFP via electrostatic interaction. The transfection efficiency of the magnetic nano-gene vector with the magnetic field was determined by a fluorescence-inverted microscope and flow cytometry. The results showed significant improvement compared with the control group (p < 0.05). The magnetic complexes also exhibited up to 6-times higher transfection efficiency compared with commonly used PEI or lipofectin. On the basis of these results, the antitumor effect with suicide gene therapy using pACTERT-TRAIL in vitro and vivo was evaluated. In vitro apoptosis was determined with the Annexin V-FITC Apoptosis Detection Kit. The results suggested that PEI-modified Fe$_{3}$O$_{4}$ nanoparticles could mediate the killing of Tca83 cells. Furthermore, treatment with pACTERT-TRAIL delivered by magnetic nanoparticles showed a significant cytostatic effect through the induction of apoptosis in a xenograft model. This indicates that magnetic nano-gene vectors could improve the transgene efficiency for Tca83 cells and could exhibit antitumor functions with the plasmid pACTERT-TRAIL. This may be a new way to treat OSCC.
Starting Page	663
Ending Page	672
Page Count	10
File Format	PDF
ISSN	10859195
Journal	Cell Biochemistry and Biophysics
Volume Number	69
Issue Number	3
e-ISSN	15590283
Language	English
Publisher	Springer US
Publisher Date	2014-02-22
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Oral squamous cell carcinoma Nanoparticles Polyethylenimine Apoptosis Biochemistry Pharmacology/Toxicology Biotechnology Cell Biology Biophysics and Biological Physics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Biochemistry Biophysics

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