NDLI: The Expression and Pharmacological Characterization of Nicotinic Acetylcholine Receptor Subunits in HBE16 Airway Epithelial Cells

Please wait, while we are loading the content...

Comparative Screening of K-ras Mutations in Colorectal Cancer and Lung Cancer Patients Using a Novel Real-Time PCR with ADx-K-ras Kit and Sanger DNA Sequencing

The Expression and Pharmacological Characterization of Nicotinic Acetylcholine Receptor Subunits in HBE16 Airway Epithelial Cells

Characterization of Escherichia coli [NiFe]-Hydrogenase Distribution During Fermentative Growth at Different pHs

Insulin Receptors and Downstream Substrates Associate with Membrane Microdomains after Treatment with Insulin or Chromium(III) Picolinate

Escherichia coli Membrane-Associated Energy-Dependent Processes and Sensitivity Toward Antibiotics Changes as Responses to Low-Intensity Electromagnetic Irradiation of 70.6 and 73 GHz Frequencies

Hypothesis: Human Umbilical Cord Blood-Derived Stromal Cells Regulate the Foxp3 Expression of Regulatory T Cells Through the TGF-β1/Smad3 Pathway

Vinorelbine-Induced Oxidative Injury in Human Endothelial Cells Mediated by AMPK/PKC/NADPH/NF-κB Pathways

Interaction of Doxorubicin and Dipalmitoylphosphatidylcholine Liposomes

PH-Induced Molten Globule State of Rhizopus niveus Lipase is More Resistant Against Thermal and Chemical Denaturation Than Its Native State

Unresolved Inflammation and Cancer: Loss of Natural Immune Surveillance as the Correct ‘Target’ for Therapy! Seeing the ‘Elephant’ in the Light of Logic

Cell Biochemistry and Biophysics : Volume 62, Issue 2, March 2012

Cell Biochemistry and Biophysics : Volume 62, Issue 1, January 2012

Cell Biochemistry and Biophysics : Volume 61

Cell Biochemistry and Biophysics : Volume 60

Cell Biochemistry and Biophysics : Volume 59

Cell Biochemistry and Biophysics : Volume 58

Cell Biochemistry and Biophysics : Volume 57

Cell Biochemistry and Biophysics : Volume 56

Cell Biochemistry and Biophysics : Volume 55

Cell Biochemistry and Biophysics : Volume 54

Cell Biochemistry and Biophysics : Volume 53

Cell Biochemistry and Biophysics : Volume 52

Cell Biochemistry and Biophysics : Volume 51

Cell Biochemistry and Biophysics : Volume 50

Cell Biochemistry and Biophysics : Volume 49

Cell Biochemistry and Biophysics : Volume 48

Cell Biochemistry and Biophysics : Volume 47

Cell Biochemistry and Biophysics : Volume 46

Cell Biochemistry and Biophysics : Volume 45

Cell Biochemistry and Biophysics : Volume 44

Cell Biochemistry and Biophysics : Volume 43

Cell Biochemistry and Biophysics : Volume 42

Cell Biochemistry and Biophysics : Volume 41

Cell Biochemistry and Biophysics : Volume 40

Cell Biochemistry and Biophysics : Volume 39

Cell Biochemistry and Biophysics : Volume 38

Cell Biochemistry and Biophysics : Volume 37

Cell Biochemistry and Biophysics : Volume 36

Cell Biochemistry and Biophysics : Volume 35

Cell Biochemistry and Biophysics : Volume 34

Cell Biochemistry and Biophysics : Volume 33

Cell Biochemistry and Biophysics : Volume 32

Cell Biochemistry and Biophysics : Volume 31

Cell Biochemistry and Biophysics : Volume 30

Cell Biochemistry and Biophysics : Volume 29

Cell Biochemistry and Biophysics : Volume 28

Cell Biochemistry and Biophysics : Volume 27

The Expression and Pharmacological Characterization of Nicotinic Acetylcholine Receptor Subunits in HBE16 Airway Epithelial Cells

Content Provider	Springer Nature Link
Author	Li, Qi Zhou, Xiangdong Kolosov, Victor P. Perelman, Juliy M.
Copyright Year	2011
Abstract	This study characterizes the expression and the biological effects of the nicotinic acetylcholine receptor (nAChR) on human airway epithelial cells. Cultured HBE16 airway epithelial cells were incubated with either nicotine or cigarette smoke extract (CSE). The nAChR gene and protein expression in cells were detected by reverse transcriptase-polymerase chain reaction (RT-PCR), real-time PCR, and western blot. The protein expression of the nAChR subunits, α1, α5, and α7, were evaluated by immunohistochemistry. Cells were subsequently transfected with α1-, α5-, and α7-specific siRNAs, and the effects of nicotine on the production of the pro-inflammatory factors, TNF-α, IL-8, and IL-6 in transfected cells were analyzed using an enzyme-linked immunosorbent assay and real-time PCR. We detected α1, α5, α7, and β2 subunits in untreated HBE16 cells, and their expression was elevated after nicotinic incubation. Importantly, the most significant increase in expression was observed in the α5 and α7 subunits. However, CSE did not cause a significant enhancement in the expression of these genes and proteins. Cells pretreated with nicotine prior to lipopolysaccharide (LPS) stimulation exhibited a lower production of TNF-α, IL-8, and IL-6 compared to LPS-treated (only) cells. Cells that were transfected with α7 siRNA and subsequently incubated with nicotine and LPS, exhibited a higher expression of TNF-α, IL-8, and IL-6 compared with non-transfected cells or α1 and α5 siRNA-transfected cells. In α1- and α5-siRNA-transfected cells, the expression of TNF-α, IL-8, and IL-6 showed no significant difference compared with non-transfected cells. Therefore, we concluded that α1, α5, α7, and β2 nAChR subunits are highly expressed in human bronchial epithelial cells (HBE16) after nicotinic incubation and that the α7 subunit is involved in the nicotine-induced inhibitory effect on the production of inflammatory factors. Moreover, α1, α5, and β2 subunits did not play an important role in this process.
Starting Page	421
Ending Page	431
Page Count	11
File Format	PDF
ISSN	10859195
Journal	Cell Biochemistry and Biophysics
Volume Number	62
Issue Number	3
e-ISSN	15590283
Language	English
Publisher	Humana Press Inc
Publisher Date	2011-11-18
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Nicotine Cigarette smoke Inflammatory factor Nicotinic acetylcholine receptors Biotechnology Cell Biology Pharmacology/Toxicology Biophysics and Biological Physics Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Biochemistry Biophysics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Heteromeric α7β2 Nicotinic Acetylcholine Receptors in the Brain

Neuronal nicotinic acetylcholine receptor expression and function on nonneuronal cells

Nicotine Craving and Withdrawal: Impact of Cigarette Smoke Constituents and Age

Distribution of the a2, a3, and a5 nicotinic acetylcholine receptor subunits in the chick brain

Melatonin modulation of presynaptic nicotinic acetylcholine receptors located on short noradrenergic neurons of the rat vas deferens: a pharmacological characterization.

Frequent occurrence of nicotinic acetylcholine receptors in GABAergic neurons of the chick visual system

Running title: nicotine alters α6β2 * nicotinic acetylcholine receptors.

Roles for N-terminal Extracellular Domains of Nicotinic Acetylcholine Receptor (nAChR) β3 Subunits in Enhanced Functional Expression of Mouse α6β2β3- and α6β4β3-nAChRs

Organelle-specific single-molecule imaging of α4β2 nicotinic receptors reveals the effect of nicotine on receptor assembly and cell-surface trafficking

The Expression and Pharmacological Characterization of Nicotinic Acetylcholine Receptor Subunits in HBE16 Airway Epithelial Cells

Similar Documents

Heteromeric α7β2 Nicotinic Acetylcholine Receptors in the Brain

Neuronal nicotinic acetylcholine receptor expression and function on nonneuronal cells

Nicotine Craving and Withdrawal: Impact of Cigarette Smoke Constituents and Age

Distribution of the a2, a3, and a5 nicotinic acetylcholine receptor subunits in the chick brain

Melatonin modulation of presynaptic nicotinic acetylcholine receptors located on short noradrenergic neurons of the rat vas deferens: a pharmacological characterization.

Frequent occurrence of nicotinic acetylcholine receptors in GABAergic neurons of the chick visual system

Running title: nicotine alters α6β2 * nicotinic acetylcholine receptors.

Roles for N-terminal Extracellular Domains of Nicotinic Acetylcholine Receptor (nAChR) β3 Subunits in Enhanced Functional Expression of Mouse α6β2β3- and α6β4β3-nAChRs

Organelle-specific single-molecule imaging of α4β2 nicotinic receptors reveals the effect of nicotine on receptor assembly and cell-surface trafficking

The Expression and Pharmacological Characterization of Nicotinic Acetylcholine Receptor Subunits in HBE16 Airway Epithelial Cells