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The effects of cardiac myocytes on interstitial fibroblasts in toxic iron overload

Content Provider	Springer Nature Link
Author	Liu, Ying Templeton, Douglas M.
Copyright Year	2001
Abstract	Iron deposits preferentially in myocytes in mixed cultures of cardiac myocytes and nonmyocytic fibroblasts. In vivo, iron overload is associated with cardiac fibrosis. Therefore, we examined whether iron loading of cardiac myocytes in culture could trigger a response in nonmyocytes characteristic of a fibrogenic phenotype. We found that the nonmyocytes adopted a myofibroblast phenotype in culture. The rate of DNA synthesis (measured by [$^{3}$H]thymidine incorporation) by the nonmyocytes was decreased by the myocyte-conditioned medium, compared to that of the unconditioned medium, and this activity was retained in >10-kDa fractions. The rate was partially restored when the medium was obtained from iron-loaded myocytes, and in this medium, the >10-kDa fraction was even more effective in reversing the suppression of proliferation. This suppression suggests a decreased secretion of a growth-inhibitory substance in the iron-loaded myocytes, and this effect was partially reversed when the iron-loaded cells were treated with the iron chelator, deferoxamine. This indicates that cardiac myocytes may play a paracrine role in suppressing the proliferation of myofibroblasts that is partially overcome when the myocytes are iron overloaded. The myocyte-conditioned medium also affects the myofibroblast phenntype, increasing the cells' fibronectin mRNA content and decreasing α-smooth-muscle actin mRNA. The myocyte-conditioned medium increases transforming growth factor-β (TGF-β) secretion by myofibroblasts, but the TGF-β content of the conditioned medium was found to play, at most, a minor role in determining the response of the myofibroblast.
Starting Page	299
Ending Page	308
Page Count	10
File Format	PDF
ISSN	15307905
Journal	Cardiovascular Toxicology
Volume Number	1
Issue Number	4
e-ISSN	15590259
Language	English
Publisher	Humana Press
Publisher Date	2001-01-01
Publisher Place	Totowa
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Iron overload iron toxicity cardiac myocytes myofibroblasts fibrosis TGF-β iron chelation Pharmacology/Toxicology Cardiology
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Toxicology Cardiology and Cardiovascular Medicine

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