NDLI: Drug Resistance of Endocardial Endothelial Cells is Related to Higher Endogenous ABCG2

Content Provider	Springer Nature Link
Author	Ajithkumar, G. S. Vinitha, A. Binil Raj, S. S. Kartha, C. C.
Copyright Year	2015
Abstract	Endocardial endothelial cells (EECs), when compared with endothelial cells of arteries and veins, possess higher resistance to apoptosis-inducing anticancer agents. The mechanism of this resistance property is unknown. We have investigated the molecular mechanism, which contributes to increased cell survival capacity in EECs. We explored whether the resistance to apoptosis is associated with the cellular expression of ATP-binding cassette transporters such as P-glycoprotein, MRP-1, and ABCG2. We used primary and immortalized porcine endocardial endothelial cells (PEECs and hTERT PEECs) and compared the results with that in porcine aortic endothelial cells (PAECs), left atrioventricular valve endothelial cells (PVECs), and human umbilical vein endothelial cell line (EA.hy926). FACS and immunoblot analysis revealed a significantly higher expression of ABCG2 in PEECs and hTERT PEECs compared to PAECs, PVECs, and EA.hy926. Using apoptosis-inducing anticancer agents such as doxorubicin and camptothecin, through chromatin condensation assay and immunoblot analysis, we demonstrated a higher resistance to apoptosis in EECs compared to PAECs, PVECs, and EA.hy926. Interestingly, resistance in EECs reversed in presence of ABCG2 specific inhibitor, fumitremorgin C. Our observations suggest that an inherently high expression of ABCG2 in EECs protects them against apoptosis in presence of anticancer agents.
Starting Page	390
Ending Page	405
Page Count	16
File Format	PDF
ISSN	15307905
Journal	Cardiovascular Toxicology
Volume Number	16
Issue Number	4
e-ISSN	15590259
Language	English
Publisher	Springer US
Publisher Date	2015-12-11
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Endocardial endothelial cells ABCG2 Apoptosis Doxorubicin Camptothecin Fumitremorgin C Pheophorbide-A Pharmacology/Toxicology Cardiology
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Toxicology Cardiology and Cardiovascular Medicine

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