NDLI: Zinc- and Copper-Induced Interleukin-6 Release in Primary Cell Cultures From Rat Heart

Content Provider	Springer Nature Link
Author	Ansteinsson, V. Refsnes, M. Skomedal, T. Osnes, J. B. Schiander, I. Låg, M.
Copyright Year	2009
Abstract	The metals, zinc (Zn$^{2+}$) and copper (Cu$^{2+}$) from inhaled particulate matter may reach the systemic circulation and the cardiac tissue. In the present study, the potential of Zn$^{2+}$ and Cu$^{2+}$ to induce interleukin (IL)-6 responses in cardiomyocytes (CMs) and cardiac fibroblasts (CFs), in mono- and cocultures, was examined. Both metals induced IL-6 release in a concentration (20–200 μM)-dependent manner. Zn$^{2+}$ appeared more potent than Cu$^{2+}$ in both mono- and cocultures of CMs and CFs. In the cocultures, the basal- and metal-induced IL-6 responses were synergistically increased compared to the monocultures. Exposure to Zn$^{2+}$ increased phosphorylation of the MAP-kinases, ERK1/2 and p38, in monocultures of CMs and CFs. Cu$^{2+}$ induced an increased phosphorylation of p38 in both cell types and of ERK1/2 in CFs, but at higher concentrations than Zn$^{2+}$. Treatment with a p38 inhibitor (SB202190) reduced the IL-6 responses to Zn$^{2+}$ and Cu$^{2+}$ in both cell types. Pretreatment with PD98059 to inhibit ERK1/2 was without significant effect; however, insignificant reductions was observed in the in the CFs. In conclusion, Zn$^{2+}$ and Cu$^{2+}$ increased IL-6 release and MAP-kinase activation in primary cardiac cells, processes known to be involved in cardiac inflammation and hypertrophy.
Starting Page	86
Ending Page	94
Page Count	9
File Format	PDF
ISSN	15307905
Journal	Cardiovascular Toxicology
Volume Number	9
Issue Number	2
e-ISSN	15590259
Language	English
Publisher	Humana Press Inc
Publisher Date	2009-06-11
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Zinc Copper Metals Cytokine Interleukin-6 MAP-kinases Cardiac cells Cocultures Cardiology Pharmacology/Toxicology
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Toxicology Cardiology and Cardiovascular Medicine

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