NDLI: Proteomic Analysis of Osteoblasts Exposed to Fluoride In Vitro

Content Provider	Springer Nature Link
Author	Xu, Hui Jing, Ling Li, Guang Sheng
Copyright Year	2007
Abstract	Proteomical analysis is defined as the characterization of the entire set of protein encoded a genome. Two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI-TOF MS) are main techniques used in proteomic analysis to achieve information about protein expression profiles. Knowledge about the mechanism of skeletal fluorosis can be gained by recognizing changes in protein expression. To better understand the skeletal fluorosis process, osteoblasts isolated from calvarial of neonatal mouse were cultured and treated with 2 ppm fluoride for 72 h, and proteins of the osteoblast were profiled by 2-DE. With the analysis of Image-Master 2D analysis software, we detected a total number of 493 matching spots on 2-DE images. Among them, 28 protein spots showed twofold significant alteration (P < 0.05) in fluoride-exposed groups. Moreover, 12 proteins were identified by MALDI-TOF MS. These identified proteins in fluoride-exposed group were associated with cell proliferation, metabolism, and oxidative folding. Thus, our study provides useful information on fluoride-related changes of proteome and shows that proteomical analysis is a powerful methodology for the better understanding of skeletal fluorosis.
Starting Page	91
Ending Page	97
Page Count	7
File Format	PDF
ISSN	01634984
Journal	Biological Trace Element Research
Volume Number	123
Issue Number	1-3
e-ISSN	15590720
Language	English
Publisher	Humana Press Inc
Publisher Date	2008-01-16
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Proteomic Osteoblast Fluorosis Skeletal fluorosis Oncology Nutrition Biotechnology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Biochemistry (medical) Endocrinology, Diabetes and Metabolism Clinical Biochemistry Biochemistry Inorganic Chemistry

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