NDLI: Reverse Micellar Extraction of β-Galactosidase from Barley (Hordeum vulgare)

Content Provider	Springer Nature Link
Author	Hemavathi, A. B. Umesh Hebbar, H. Raghavarao, K. S. M. S.
Copyright Year	2008
Abstract	The reverse micellar system of sodium bis(2-ethylhexyl)sulfosuccinate (AOT)/isooctane was used for the extraction and primary purification of β-galactosidase (EC 3.2.1.23) from the aqueous extract of barley (Hordeum vulgare) for the first time. The process parameters such as the concentration of the surfactant, the volume of the sample injected, and its protein concentration, pH, and ionic strength of the initial aqueous phase for forward extraction, buffer pH, and salt concentration for back extraction are varied to optimize the extraction efficiency. Studies carried out with both phase transfer and injection mode of reverse micellar extraction confirmed the injection mode to be more suitable for β-galactosidase extraction. The extent of reverse micellar solubilization of proteins increased with an increase in protein concentration of the feed sample. However, back extraction efficiency remained almost constant (13–14.4%), which indicates the selectivity of AOT reverse micelles for a particular protein under given experimental conditions. β-Galactosidase was extracted with an activity recovery of 98.74% and a degree of purification of 7.2-fold.
Starting Page	522
Ending Page	531
Page Count	10
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	151
Issue Number	2-3
e-ISSN	15590291
Language	English
Publisher	Humana Press Inc
Publisher Date	2008-05-15
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Reverse micellar system Barley β-Galactosidase Phase transfer Injection mode Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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