NDLI: Diatom-Based Label-Free Optical Biosensor for Biomolecules

Content Provider	Springer Nature Link
Author	Viji, S. Anbazhagi, M. Ponpandian, N. Mangalaraj, D. Jeyanthi, S. Santhanam, P. Devi, A. Shenbaga Viswanathan, C.
Copyright Year	2014
Abstract	Diatoms are unicellular algae, which fabricates ornate biosilica shells called frustules that possess a surface rich in reactive silanol (Si–OH) groups. The intrinsic patterned porous structure of diatom frustules at nanoscale can be exploited in the effective detection of biomolecules. In this study, the frustules of a specific diatom Amphora sp. has been functionalized to detect bovine serum albumin (BSA). The functionalization of the diatom frustule substrate is achieved by using 3-aminopropyltriethoxysilane (APES). The field emission scanning electron microscopy (FESEM) results showed an ornately patterned surface of the frustule valve ordered at nanoscale. The Fourier transform infrared (FTIR) spectra confirmed the N–H bending and stretching of the amine group after amine functionalization. The emission peaks in the photoluminescence (PL) spectra of the amine-functionalized diatom biosilica selectively enhanced the intensity by a factor of ten when compared to that of a bare diatom biosilica. The result showed a significant quenching of PL intensity of BSA at around 445 nm due to the interaction of amine-functionalized diatom–BSA protein complex. The detection limit was found to be 3 × 10$^{−5}$ M of BSA protein. Hence, the study proves that the functionalized frustule of Amphora sp. is an effective quantitative analytical tool for optical label-free biosensing applications.
Starting Page	1166
Ending Page	1173
Page Count	8
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	174
Issue Number	3
e-ISSN	15590291
Language	English
Publisher	Springer US
Publisher Date	2014-07-03
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Diatom Amphora sp Biosensing Photoluminescence Bovine serum albumin Biotechnology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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