NDLI: The effect of glucose on high-level xylose fermentations by recombinant Zymomonas in batch and fed-batch fermentations

Content Provider	Springer Nature Link
Author	Lawford, Hugh G. Rousseau, Joyce D.
Copyright Year	1999
Abstract	Xylose-fermenting recombinant Zymomonas mobilis has been proposed as a candidate biocatalyst for the production of fuel ethanol from cellulosic biomass and wastes. This study documents the effect of glucose on xylose utilization by recombinant Z. mobilis CP4:pZB5 using a nutrient-rich synthetic (puresugar) hardwood dilute-acid prehydrolyzate medium containing 0.8% (w/v) glucose and 4% (w/v) xylose that was enriched with respect to xylose concentration within the range 6–10% (w/v) xylose. Supplementation with glucose toafinal concentration of 2% (w/v) resulted in faster xylose utilization of both 6% and 8% xylose; however, higher levels of glucose supplementation (>2%) did not result in a decrease in the time required for fermentation of either 6% or 8% xylose. An improvement in the rate of 8% xylose utilization was also achieved through, continuous glucose feeding in which the total glucose concentration was about 1.3% (w/v). This fedbatch experiment was designed to mimic the continuous supply of glucose provided by the cellulose saccharifying enzymes in a simultaneous saccharifying and cofermentation process. The upper limit ethanol concentration at which xylose utilization by recombinant Z. mobilis CP4:pZB5 is completely inhibited is about 5.5% (w/v) at pH 5 and >6% at pH 5.75. At pH 5.75, this level of ethanol was achieved with the following media of pure sugar mixtures (each containing the same sugar loading of 12% (w/v): 1. 6% xylose+6% glucose; 2. 8% xylose+4% glucose; and 3. 4% xylose+8% glucose. At the level of inoculum used in this study, complete fermentation of the 12% sugar mixtures required 2–3 d (equivalent to a volumetric ethanol productivity of 0.83–1.25 g ethanol/L.h). The sugar-to-ethanol conversion efficiency was 94–96% of theoretical maximum.
Starting Page	235
Ending Page	249
Page Count	15
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	77
Issue Number	1-3
e-ISSN	15590291
Language	English
Publisher	Humana Press
Publisher Date	1999-01-01
Publisher Place	Totowa
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biotechnology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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