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| Content Provider | Springer Nature Link |
|---|---|
| Author | Matsumoto, Mitsufumi Sugiyama, Hiroshi Maeda, Yoshiaki Sato, Reiko Tanaka, Tsuyoshi Matsunaga, Tadashi |
| Copyright Year | 2009 |
| Abstract | Marine diatom, strain JPCC DA0580, and marine green microalga strain NKG400014 were selected as high neutral lipid-producers from marine microalgal culture collection toward biodiesel production. These strains were tentatively identified as Navicula sp. and Chlorella sp., respectively, by 18S rDNA analysis. Growth and lipid accumulation conditions of both strains were analyzed by changing nutrient concentrations in growth media and initial illuminance intensity. The highest productivity of fatty acid methyl ester (FAME) reached to 154 mg/L/week for NKG400014 and 185 mg/L/week for JPCC DA0580. Gas chromatography/mass spectrometry analysis indicates that FAME fraction from NKG400014 mainly contained 9-12-15-octadecatrienoate (C18:3) and that from JPCC DA0580 mainly contained methyl palmitate (C16:0) and methyl palmitoleate (C16:1). Furthermore, calorimetric analysis revealed that the energy content of strain was 4,233 ± 55 kcal/kg (i.e., 15.9 ± 0.2 MJ/kg) for NKG400014 and 6,423 ± 139 kcal/mg (i.e., 26.9 ± 0.6 MJ/kg) for JPCC DA0580, respectively. The value from JPCC DA0580 was equivalent to that of coal. The strains NKG400014 and JPCC DA0580 will become a promising resource that can grow as dominant species in the open ocean toward production of both liquid and solid biofuels. |
| Starting Page | 483 |
| Ending Page | 490 |
| Page Count | 8 |
| File Format | |
| ISSN | 02732289 |
| Journal | Applied Biochemistry and Biotechnology |
| Volume Number | 161 |
| Issue Number | 1-8 |
| e-ISSN | 15590291 |
| Language | English |
| Publisher | Humana Press Inc |
| Publisher Date | 2009-09-08 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biodiesel Triglyceride Fatty acid methyl ester Microalgae Calorimetry Biochemistry Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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