NDLI: Cloning and Expression Characteristics of the Notch-Associated Gene BmE(spl)mγ from Silkworm, Bombyx mori

Content Provider	Springer Nature Link
Author	Liu, Min Wang, Chan Li, Dan Liu, Yue Sheng, Qing Lv, Zhengbing Yu, Wei Wang, Dan Zhang, Yaozhou Nie, Zuoming
Copyright Year	2014
Abstract	The E(spl)mγ gene in Drosophila is a regulatory target gene downstream of the Notch pathway. BmE(spl)mγ (Bombyx mori, E(spl)mγ) is an ortholog of the Drosophila E(spl)mγ gene, and the gene encodes a protein with 248 amino acid residues. This gene was cloned and overexpressed in Escherichia coli BL21(DE3). The recombinant protein was purified and subsequently used to generate a rabbit polyclonal antibody. Western blotting analyses showed that BmE(spl)mγ expression is high in pupa and egg, and low in larva and moth. In the fifth instar larva, the protein levels are high in head, epidermis, sexual gland, trachea, and the fatbody and low in the Malpighian tubule, hemolymph, gut, and silk gland. The further immunohistochemical analyses also showed higher BmE(spl)mγ expression in the head of fifth instar larva and pupa. Of the four moth parts studied, the thorax had the highest expression level. Thus, BmE(spl)mγ might be associated with neurogenesis in silkworm. Furthermore, DAPT (a γ-secretase inhibitor and an indirect inhibitor of Notch) blocking experiments showed that higher concentrations of the blocking agent and a longer processing time reduce the transcription levels of the BmE(spl)mγ gene, demonstrating that the silkworm BmE(spl)mγ gene is associated with the Notch signal pathway. These findings suggest that the function of BmE(spl)mγ may be similar to that of its Drosophila homolog.
Starting Page	2065
Ending Page	2075
Page Count	11
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	173
Issue Number	8
e-ISSN	15590291
Language	English
Publisher	Springer US
Publisher Date	2014-06-11
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	BmE(spl)mγ Notch signal pathway Expression profiling Immunohistochemistry Subcellular localization Biotechnology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Expression Analysis and Characteristics of Profilin Gene from Silkworm, Bombyx mori

Cloning and expression characteristics of the notch-associated gene BmE(spl)mÎ³ from silkworm, Bombyx mori.

Subcellular Localization and RNA Interference of an RNA Methyltransferase Gene from Silkworm, Bombyx Mori

Heterologous Expression Characteristics of Trichoderma viride Endoglucanase V in the Silkworm, Bombyx mori L.

Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori

Human insulin gene expressing with Bombyx mori multiple nucleopolyhedrovirus (BmMNPV) expression system

Expression and subcellular localization of a novel gene Bm-X of the silkworm, Bombyx mori

CDNA cloning and mRNA expression of LIM protein gene homologue from the silkworm, Bombyx mori

CDNA cloning and mRNA expression of l-3,4-dihydroxyphenylalanine decarboxylase gene homologue from the silkworm, Bombyx mori

Cloning and Expression Characteristics of the Notch-Associated Gene BmE(spl)mγ from Silkworm, Bombyx mori

Similar Documents

Expression Analysis and Characteristics of Profilin Gene from Silkworm, Bombyx mori

Cloning and expression characteristics of the notch-associated gene BmE(spl)mÎ³ from silkworm, Bombyx mori.

Subcellular Localization and RNA Interference of an RNA Methyltransferase Gene from Silkworm, Bombyx Mori

Heterologous Expression Characteristics of Trichoderma viride Endoglucanase V in the Silkworm, Bombyx mori L.

Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori

Human insulin gene expressing with Bombyx mori multiple nucleopolyhedrovirus (BmMNPV) expression system

Expression and subcellular localization of a novel gene Bm-X of the silkworm, Bombyx mori

CDNA cloning and mRNA expression of LIM protein gene homologue from the silkworm, Bombyx mori

CDNA cloning and mRNA expression of l-3,4-dihydroxyphenylalanine decarboxylase gene homologue from the silkworm, Bombyx mori

Cloning and Expression Characteristics of the Notch-Associated Gene BmE(spl)mγ from Silkworm, Bombyx mori