NDLI: Magnetic Bead Cellulose as a Suitable Support for Immobilization of α-Chymotrypsin

Content Provider	Springer Nature Link
Author	Prikryl, Petr Lenfeld, Jiri Horak, Daniel Ticha, Marie Kucerova, Zdenka
Copyright Year	2012
Abstract	Magnetic bead cellulose was prepared by a suspension method from the mixture of viscose and magnetite using thermal sol–gel transition and regeneration of cellulose. The prepared magnetic particles after their activation with divinyl sulfone were shown to be suitable magnetic carrier for immobilization of α-chymotrypsin and for its application in proteomic studies. The specific activity of the immobilized proteinase was high; its activity did not change in the course of storage. The following properties of the immobilized proteinase were compared with those of the soluble enzyme: pH and temperature dependence of the activity, self-cleavage activity, and possibility of repeated use. α-Chymotrypsin immobilized to magnetic bead cellulose was used for the proteolytic digestion of porcine pepsin A and human gastric juice and a possibility of direct use of enzyme reaction products for matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis was shown.
Starting Page	295
Ending Page	305
Page Count	11
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	168
Issue Number	2
e-ISSN	15590291
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-06-27
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Enzyme immobilization Human gastric juice Immobilized α-chymotrypsin Magnetic bead cellulose Porcine pepsin A Biotechnology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Efficient Immobilization of Porcine Pancreatic α-Amylase on Amino-Functionalized Magnetite Nanoparticles: Characterization and Stability Evaluation of the Immobilized Enzyme

Influence of Water Miscible Organic Solvents on α-chymotrypsin in Solution and Immobilized on Eupergit CM

Expression, One-Step Purification, and Immobilization of HaloTag$^{TM}$ Fusion Proteins on Chloroalkane-Functionalized Magnetic Beads

Bacterial cellulose-chitosan composite hydrogel beads for enzyme immobilization

Application of magnetic nanoparticles in smart enzyme immobilization

High transesterification activities of immobilized proteases in new ether-functionalized ionic liquids

A Review: Potential Usage of Cellulose Nanofibers (CNF) for Enzyme Immobilization via Covalent Interactions

Immobilization of β-Galactosidase onto Magnetic Beads

Functionalized Magnetic Bacterial Cellulose Beads as Carrier for Lecitase® Ultra Immobilization

Magnetic Bead Cellulose as a Suitable Support for Immobilization of α-Chymotrypsin

Similar Documents

Efficient Immobilization of Porcine Pancreatic α-Amylase on Amino-Functionalized Magnetite Nanoparticles: Characterization and Stability Evaluation of the Immobilized Enzyme

Influence of Water Miscible Organic Solvents on α-chymotrypsin in Solution and Immobilized on Eupergit CM

Expression, One-Step Purification, and Immobilization of HaloTag$^{TM}$ Fusion Proteins on Chloroalkane-Functionalized Magnetic Beads

Bacterial cellulose-chitosan composite hydrogel beads for enzyme immobilization

Application of magnetic nanoparticles in smart enzyme immobilization

High transesterification activities of immobilized proteases in new ether-functionalized ionic liquids

A Review: Potential Usage of Cellulose Nanofibers (CNF) for Enzyme Immobilization via Covalent Interactions

Immobilization of β-Galactosidase onto Magnetic Beads

Functionalized Magnetic Bacterial Cellulose Beads as Carrier for Lecitase® Ultra Immobilization

Magnetic Bead Cellulose as a Suitable Support for Immobilization of α-Chymotrypsin