NDLI: Embelin (2,5-Dihydroxy-3-undecyl-p-benzoquinone) for Photodynamic Therapy: Study of Their Cytotoxicity in Cancer Cells

Content Provider	Springer Nature Link
Author	Joy, Beena Kumar, S. N. Radhika, A. R. Abraham, Annie
Copyright Year	2014
Abstract	Photodynamic therapy (PDT) involves the use of photochemical reactions mediated through the interaction of photosensitizing agent for the treatment of malignant tumor. The present study was carried out to evaluate the photosensitizing potential of embelin isolated from Embelia ribes. For PDT study, cells were grouped into four groups. Two types of control were used, one with Ehrlich’s ascites carcinoma (EAC) cells alone (group 1) and one which received the illumination alone (group 2). Group 3 contains cells treated with different concentrations of embelin for 1 h at 37 °C. Another set of cells (group 4) after treatment with different concentrations of embelin was illuminated in ice for 4 min with a 1000-W halogen lamp. To study the mechanism of cell death, the following parameters such as reactive oxygen species (ROS), caspase-3, lactate dehydrogenase (LDH), and thiobarbituric acid reactive substances (TBARS) were analyzed both in the absence and presence of light after the treatment of embelin. PDT study clearly indicated that embelin alone recorded no cytotoxicity, but for light treatment alone with the different concentrations of embelin, there was a significant induction of cytotoxicity in a concentration-dependent manner. Level of ROS and LDH increased in cells treated with embelin. Moreover, caspase-3 also increased which clearly indicated that cell death is through caspase-dependent apoptosis. This is the first report on PDT, a novel modality, using embelin for cancer therapy in vitro. The novelty of the study depends on the fact that cytotoxicity was produced by the synergistic effect of the embelin and light.
Starting Page	1069
Ending Page	1079
Page Count	11
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	175
Issue Number	2
e-ISSN	15590291
Language	English
Publisher	Springer US
Publisher Date	2014-10-31
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Embelin (2,5-dihydroxy-3-undecyl-p-benzoquinone) Embelia ribes Ehrlich’s ascites carcinoma Photosensitizers Phototoxicity Biotechnology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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