NDLI: Adaptive Evolution of Escherichia coli Inactivated in the Phosphotransferase System Operon Improves Co-utilization of Xylose and Glucose Under Anaerobic Conditions

Content Provider	Springer Nature Link
Author	Balderas Hernández, Victor Emmanuel Hernández Montalvo, Verónica Bolívar, Francisco Gosset, Guillermo Martínez, Alfredo
Copyright Year	2010
Abstract	Modification of the phosphoenolpyruvate/sugar phosphotransferase system (PTS) has shown improvement in sugar coassimilation in Escherichia coli production strains. However, in preliminary experiments under anaerobic conditions, E. coli strains with an inactive PTS and carrying pLOI1594, which encodes pyruvate decarboxylase and alcohol dehydrogenase from Zymomonas mobilis, were unable to grow. These PTS$^{−}$ strains were previously evolved under aerobic conditions to grow rapidly in glucose (PTS$^{-}$ Glucose$^{+}$ phenotype). Thus, in this work, applying a continuous culture strategy under anaerobic conditions, we generate a new set of evolved PTS$^{−}$ Glucose$^{+}$ mutants, VH30N1 to VH30N6. Contrary to aerobically evolved mutants, strains VH30N2 and VH30N4 carrying pLOI1594 grew in anaerobiosis; also, their growth capacity was restored in a 100%, showing specific growth rates (μ ~ 0.12 h$^{−1}$) similar to the PTS$^{+}$ parental strain (μ = 0.11 h$^{−1}$). In cultures of VH30N2/pLOI1594 and VH30N4/pLOI1594 using a glucose–xylose mixture, xylose was totally consumed and consumption of sugars occurred in a simultaneous manner indicating that catabolic repression is alleviated in these strains. Also, the efficient sugar coassimilation by the evolved strains caused an increment in the ethanol yields.
Starting Page	485
Ending Page	496
Page Count	12
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	163
Issue Number	4
e-ISSN	15590291
Language	English
Publisher	Humana Press Inc
Publisher Date	2010-08-26
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Anaerobiosis Evolved strains PTS Sugar co-utilization Xylose Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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