NDLI: Extraction of Proteins from Switchgrass Using Aqueous Ammonia within an Integrated Biorefinery

Content Provider	Springer Nature Link
Author	Bals, Bryan Teachworth, Laura Dale, Bruce Balan, Venkatesh
Copyright Year	2007
Abstract	Switchgrass (Panicum vergatum) is a potential feedstock for future cellulosic biorefineries. Such a feedstock may also provide protein, most likely for use as an animal feed. In this paper, we present a potential scheme for integrating fiber processing with extractions to obtain both sugar and protein products from switchgrass pretreated using Ammonia Fiber Expansion (AFEX). Solutions of 3% aqueous ammonia at pH 10.5 provided optimal extraction of proteins. Addition of the nonionic surfactant Tween-80 improved protein recovery for AFEX-treated materials. It was determined that an extraction following AFEX solubilized approximately 40% of the protein, while a subsequent hydrolysis solubilized much of the remaining protein while producing 325 g sugar per kg biomass. The remaining insoluble residue contained very little protein or ash, making it ideal for heat and power production. In contrast, an extraction following hydrolysis solubilized only 68% of the original protein in the biomass, while obtaining slightly higher sugar yields.
Starting Page	187
Ending Page	198
Page Count	12
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	143
Issue Number	2
e-ISSN	15590291
Language	English
Publisher	Humana Press Inc
Publisher Date	2007-06-22
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	AFEX Switchgrass Extraction Enzymatic hydrolysis Leaf protein Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Protein Extraction and Enzymatic Hydrolysis of Ammonia-Treated Cassava Leaves (Manihot esculenta Crantz)

Pretreatment of switchgrass by ammonia fiber explosion (AFEX)

Pretreatment with ammonia water for enzymatic hydrolysis of corn husk, bagasse, and switchgrass

Ammonia Fiber Expansion (AFEX) Pretreatment, Enzymatic Hydrolysis, and Fermentation on Empty Palm Fruit Bunch Fiber (EPFBF) for Cellulosic Ethanol Production

Pilot-Scale Fermentation of Aqueous-Ammonia-Soaked Switchgrass

Hydrolyzabilities of Different Corn Stover Fractions After Aqueous Ammonia Pretreatment

Aqueous Ammonia Soaking of Switchgrass Followed by Simultaneous Saccharification and Fermentation

Addition of Surfactants and Non-Hydrolytic Proteins and Their Influence on Enzymatic Hydrolysis of Pretreated Sugarcane Bagasse

Enzymatic hydrolysis of ammonia-treated rice straw

Extraction of Proteins from Switchgrass Using Aqueous Ammonia within an Integrated Biorefinery

Similar Documents

Protein Extraction and Enzymatic Hydrolysis of Ammonia-Treated Cassava Leaves (Manihot esculenta Crantz)

Pretreatment of switchgrass by ammonia fiber explosion (AFEX)

Pretreatment with ammonia water for enzymatic hydrolysis of corn husk, bagasse, and switchgrass

Ammonia Fiber Expansion (AFEX) Pretreatment, Enzymatic Hydrolysis, and Fermentation on Empty Palm Fruit Bunch Fiber (EPFBF) for Cellulosic Ethanol Production

Pilot-Scale Fermentation of Aqueous-Ammonia-Soaked Switchgrass

Hydrolyzabilities of Different Corn Stover Fractions After Aqueous Ammonia Pretreatment

Aqueous Ammonia Soaking of Switchgrass Followed by Simultaneous Saccharification and Fermentation

Addition of Surfactants and Non-Hydrolytic Proteins and Their Influence on Enzymatic Hydrolysis of Pretreated Sugarcane Bagasse

Enzymatic hydrolysis of ammonia-treated rice straw

Extraction of Proteins from Switchgrass Using Aqueous Ammonia within an Integrated Biorefinery