NDLI: Characterization of a Novel Xylanase Gene from Rumen Content of Hu Sheep

Content Provider	Springer Nature Link
Author	Wang, Qian Luo, Yang He, Bo Jiang, Lin Shu Liu, Jian Xin Wang, Jia Kun
Copyright Year	2015
Abstract	A novel xylanase gene, xyn-lxy, was cloned from a metagenomic fosmid library, which was previously constructed from the rumen contents of Hu sheep and was functionally characterized in Escherichia coli. The open reading frame was composed of 1923 bp and encoded for 640 amino acids, including a catalytic domain of glycosyl hydrolase family 10 and carbohydrate-binding module 9. The gene showed 97 % identity with uncultured bacterium Contig1552 but low similarity with xylanases from known cellulolytic-degrading microorganisms in the rumen. The recombinant XYN-LXY showed a specific activity of 664.7 U mg$^{−1}$. The optimal temperature and pH of the enzyme were 50 °C and 6.0, respectively. Specifically, XYN-LXY was exclusively activated by Mn$^{2+}$ among all of the cations and reducing agents tested in this study. An enzymatic hydrolysis assay revealed that XYN-LXY degraded birchwood xylan into xylooligosaccharide with a low degree of polymerization. After incubation for 4 h, the concentration of the dominant product, xylobiose, was 2.297 ± 0.175 mg ml$^{−1}$ (74.07 % of total product) followed by xylose with a concentration of 0.656 ± 0.010 mg ml$^{−1}$ (21.14 % of total product). The XYN-LXY exhibited deep degradation effects on the xylan substrate, which were rarely observed with endo-xylanase, making it a promising candidate for industrial application, especially in biofuel production.
Starting Page	1424
Ending Page	1436
Page Count	13
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	177
Issue Number	7
e-ISSN	15590291
Language	English
Publisher	Springer US
Publisher Date	2015-09-10
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Rumen Xylanase Enzyme properties Hydrolysis Xylooligosaccharide Biotechnology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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