NDLI: A Newly Isolated Pseudomonas sp., Epibiotic on the Seaweed, Padina tetrastromatica, off Southeastern Coast of India, Reveals Antibacterial Action

Content Provider	Springer Nature Link
Author	Ravisankar, Aishwarya Gnanambal, Mary Elizabeth K. Sundaram, Lakshmi R.
Copyright Year	2013
Abstract	Epibionts from the red (Hypnea valentiae) and brown seaweeds (Padina tetrastromatica) were rapidly isolated on Zobell agar medium. All the isolates from both the seaweeds (76 numbers) were tested against five human pathogens which were resistant to at least one of the commercially available antibiotics at a minimal concentration of 10 mg. The most antibiotic productive isolate (PT19) from Padina tetrastromatica was extracted and observed to inhibit Klebsiella pneumoniae and Pseudomonas aeruginosa with zone sizes of 15 and 10 mm radius, respectively, at a concentration of 300 μg. Further, a direct bioautography was done and an inhibition was witnessed against the aforementioned pathogens even at 2 μg concentration around three spots (R $_{f}$ values 0.6, 0.7, and 0.8). Preparative thin-layer chromatography yielded a yellow sticky compound (6 mg) which was identified as an alkaloid. The compound on reversed-phase high-pressure liquid chromatography analysis yielded two major and two minor peaks with retention times, 3.1, 4.2, 4.7, and 4.9 min, respectively. The antibacterial compound was recorded 96.6 % pure, and the producer strain was identified as Pseudomonas sp. To our knowledge, we are the first to isolate and identify Pseudomonas from Padina tetrastromatica producing antibacterial alkaloids. This study will pave way for exploring more bacterial load from the said algal groups for bioactivities.
Starting Page	1968
Ending Page	1985
Page Count	18
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	171
Issue Number	8
e-ISSN	15590291
Language	English
Publisher	Springer US
Publisher Date	2013-09-08
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Padina tetrastromatica Macroalgae Antibacterial Alkaloid Biotechnology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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