NDLI: Production of d-tagatose, a Functional Sweetener, Utilizing Alginate Immobilized Lactobacillus fermentum CGMCC2921 Cells

Content Provider	Springer Nature Link
Author	Xu, Zheng Li, Sha Fu, Fenggen Li, Guixiang Feng, Xiaohai Xu, Hong Ouyang, Pingkai
Copyright Year	2011
Abstract	d-tagatose is a ketohexose that can be used as a novel functional sweetener in foods, beverages, and dietary supplements. This study was aimed at developing a high-yielding d-tagatose production process using alginate immobilized Lactobacillus fermentum CGMCC2921 cells. For the isomerization from d-galactose into d-tagatose, the immobilized cells showed optimum temperature and pH at 65 °C and 6.5, respectively. The alginate beads exhibited a good stability after glutaraldehyde treatment and retained 90% of the enzyme activity after eight cycles (192 h at 65 °C) of batch conversion. The addition of borate with a molar ratio of 1.0 to d-galactose led to a significant enhancement in the d-tagatose yield. Using commercial β-galactosidase and immobilized L. fermentum cells, d-tagatose was successfully obtained from lactose after a two-step biotransformation. The relatively high conversion rate and productivity from d-galactose to d-tagatose of 60% and 11.1 g l$^{−1}$ h$^{−1}$ were achieved in a packed-bed bioreactor. Moreover, lactobacilli have been approved as generally recognized as safe organisms, which makes this L. fermentum strain an attracting substitute for recombinant Escherichia coli cells among d-tagatose production progresses.
Starting Page	961
Ending Page	973
Page Count	13
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	166
Issue Number	4
e-ISSN	15590291
Language	English
Publisher	Humana Press Inc
Publisher Date	2011-12-28
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	d-Tagatose Lactobacillus fermentum Immobilized cells Packed-bed bioreactor l-arabinose isomerase Biotechnology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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