NDLI: Cloning, Expression, and Cell Localization of a Novel Small Heat Shock Protein Gene: BmHSP25.4

Content Provider	Springer Nature Link
Author	Sheng, Qing Xia, Jiayin Nie, Zuoming Zhang, Yaozhou
Copyright Year	2010
Abstract	Using molecular approaches, a new member of the Bombyx mori small heat shock protein family was cloned and characterized. The isolated gene contains an open reading frame of 672 bp, encodes a polypeptide of 223 amino acid residues with a predicted molecular mass of 25.4 kDa, and is therefore named BmHSP25.4. The gene codes for a protein homologous to the previously characterized HSP20.4 and HSP19.9. Western blotting analysis revealed that BmHSP25.4 existed in the fifth-instar larva’s fatty body and blood tissues. Immunohistochemistry assay also showed that BmHSP25.4 was located in the fifth-instar larva’s fatty body. The results of above studies have indicated constitutive expression of BmHSP25.4 in fatty body, blood tissues, and Bm5 cells. Finally, we examined the effect of heat stress on localization of BmHSP25.4 using anti-BmHSP25.4 polyclonal antibody by immunofluorescence. Under normal conditions, BmHSP25.4 was mostly found in the cytoplasm. However, after heat treatment, most of BmHSP25.4 distributed in the cell membrane. After 3 h of recovery following the heat shock treatment, the localization of BmHSP25.4 was the same as that under normal conditions.
Starting Page	1297
Ending Page	1305
Page Count	9
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	162
Issue Number	5
e-ISSN	15590291
Language	English
Publisher	Humana Press Inc
Publisher Date	2010-01-14
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	BmHSP25.4 Prokaryotic expression Purification Polyclonal antibody preparation Tissue localization Subcellular localization Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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