NDLI: Genetically Engineered Epidermal Growth Factor Conjugate Crosses Cell Membrane

Content Provider	Springer Nature Link
Author	Zhao, Baoquan Guo, Yanru Fu, Ailing
Copyright Year	2012
Abstract	Epidermal growth factor (EGF) is a well-known pleiotropic growth factor in mammal, and has been attempted to be used in many different fields. However, the application of EGF is limited because of its poor cell permeation. In order to increase membrane permeation ability of EGF, a genetically modified recombinant EGF (GST-TAT-EGF) was prepared through conjugation of EGF with two protein transduction domains, glutathione-S-transferase and TAT47-57. The results showed that the GST-TAT-EGF fusion protein exhibited higher ability in biomembrane penetration than that of the EGF alone. The results also implied that two different mechanisms (EGF receptor-mediated endocytosis and direct penetration) might be involved in GST-TAT-EGF transmembrane delivery.
Starting Page	1463
Ending Page	1471
Page Count	9
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	166
Issue Number	6
e-ISSN	15590291
Language	English
Publisher	Humana Press Inc
Publisher Date	2012-01-13
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Epidermal growth factor Membrane penetration Protein transduction domains Cell proliferation Transmembrane delivery Biotechnology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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Genetically Engineered Epidermal Growth Factor Conjugate Crosses Cell Membrane