NDLI: An Effective and Simplified Fed-Batch Strategy for Improved 2,3-Butanediol Production by Klebsiella oxytoca

Content Provider	Springer Nature Link
Author	Nie, Zhi Kui Ji, Xiao Jun Huang, He Du, Jun Li, Zhi Yong Qu, Liang Zhang, Qi Ouyang, Ping Kai
Copyright Year	2010
Abstract	Substrate concentration in 2,3-butanediol (2,3-BD) fermentation could not be controlled well in traditional feeding strategies, such as constant, impulse, and exponential feeding strategies. In the present study, fermentative 2,3-BD production by Klebsiella oxytoca was investigated under different batch and fed-batch strategies. The glucose-feedback fed-batch strategy was proved to be not effective for economical 2,3-BD production for the inability of timely feeding, leading that the bacteria reused 2,3-BD as carbon source for cell growth. Based on the phenomena that the byproducing acids caused the pH declining and the requirement of maintaining the pH at a proper level for both cell growth and 2,3-BD accumulation, an improved strategy of pH-stat fed-batch culture with glucose and sodium hydrate fed at the same time was established. Thus, the residual glucose concentration could be controlled through the adjustment of pH automatically. At last, efficient 2,3-BD production was fulfilled under this fed-batch strategy, and the highest 2,3-BD concentration, productivity, and yield were 127.9 g/l, 1.78 g/(l•h), and 0.48 g/g (2,3-BD/glucose), respectively, compared to 98.5 g/l, 1.37 g/(l•h), and 0.43 g/g obtained in glucose-feedback fed-batch strategy. This feeding strategy was simple and easy to operate and could be feasible for industrial 2,3-BD production in the future.
Starting Page	946
Ending Page	953
Page Count	8
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	163
Issue Number	8
e-ISSN	15590291
Language	English
Publisher	Humana Press Inc
Publisher Date	2010-10-13
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	2,3-Butanediol Klebsiella oxytoca pH-stat Fed-batch Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

An effective and simplified fed-batch strategy for improved 2,3-butanediol production by Klebsiella oxytoca.

Selective Production of 2,3-Butanediol and Acetoin by a Newly Isolated Bacterium Klebsiella oxytoca M1

Enhanced 2,3-butanediol production by altering the mixed acid fermentation pathway in Klebsiella oxytoca

Influence of Blocking of 2,3-Butanediol Pathway on Glycerol Metabolism for 1,3-Propanediol Production by Klebsiella oxytoca

Enhanced production of (R,R)-2,3-butanediol by metabolically engineered Klebsiella oxytoca

Efficient 2,3-butanediol production from whey powder using metabolically engineered Klebsiella oxytoca

Influence of Vitreoscilla hemoglobin gene expression on 2,3-butanediol production in Klebsiella oxytoca

In silico aided metabolic engineering of Klebsiella oxytoca and fermentation optimization for enhanced 2,3-butanediol production

Efficient 1,3-propanediol Production by Fed-Batch Culture of Klebsiella Pneumoniae: The Role of pH Fluctuation

An Effective and Simplified Fed-Batch Strategy for Improved 2,3-Butanediol Production by Klebsiella oxytoca

Similar Documents

An effective and simplified fed-batch strategy for improved 2,3-butanediol production by Klebsiella oxytoca.

Selective Production of 2,3-Butanediol and Acetoin by a Newly Isolated Bacterium Klebsiella oxytoca M1

Enhanced 2,3-butanediol production by altering the mixed acid fermentation pathway in Klebsiella oxytoca

Influence of Blocking of 2,3-Butanediol Pathway on Glycerol Metabolism for 1,3-Propanediol Production by Klebsiella oxytoca

Enhanced production of (R,R)-2,3-butanediol by metabolically engineered Klebsiella oxytoca

Efficient 2,3-butanediol production from whey powder using metabolically engineered Klebsiella oxytoca

Influence of Vitreoscilla hemoglobin gene expression on 2,3-butanediol production in Klebsiella oxytoca

In silico aided metabolic engineering of Klebsiella oxytoca and fermentation optimization for enhanced 2,3-butanediol production

Efficient 1,3-propanediol Production by Fed-Batch Culture of Klebsiella Pneumoniae: The Role of pH Fluctuation

An Effective and Simplified Fed-Batch Strategy for Improved 2,3-Butanediol Production by Klebsiella oxytoca