NDLI: A Novel Glutamate Transport System in Poly(γ-Glutamic Acid)-Producing Strain Bacillus subtilis CGMCC 0833

Content Provider	Springer Nature Link
Author	Wu, Qun Xu, Hong Zhang, Dan Ouyang, Pingkai
Copyright Year	2011
Abstract	Bacillus subtilis CGMCC 0833 is a poly(γ-glutamic acid) (γ-PGA)-producing strain. It has the capacity to tolerate high concentration of extracellular glutamate and to utilize glutamate actively. Such a high uptake capacity was owing to an active transport system for glutamate. Therefore, a specific transport system for l-glutamate has been observed in this strain. It was a novel transport process in which glutamate was symported with at least two protons, and an inward-directed sodium gradient had no stimulatory effect on it. K $_{m}$ and V $_{m}$ for glutamate transport were estimated to be 67 μM and 152 nmol$^{−1}$ min$^{−1}$ mg$^{−1}$ of protein, respectively. The transport system showed structural specificity and stereospecificity and was strongly dependent on extracellular pH. Moreover, it could be stimulated by Mg$^{2+}$, NH 4 + , and Ca$^{2+}$. In addition, the glutamate transporter in this strain was studied at the molecular level. As there was no important mutation of the transporter protein, it appeared that the differences of glutamate transporter properties between this strain and other B. subtilis strains were not due to the differences of the amino acid sequence and the structure of transporter protein. This is the first extensive report on the properties of glutamate transport system in γ-PGA-producing strain.
Starting Page	1431
Ending Page	1443
Page Count	13
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	164
Issue Number	8
e-ISSN	15590291
Language	English
Publisher	Humana Press Inc
Publisher Date	2011-03-26
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bacillus subtilis CGMCC 0833 Glutamate transport Poly(γ-glutamic acid) transport system Biotechnology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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